- 108A2 (See other available formats)
- Regulatory Status
- Other Names
- Lgals9, Gal-9, Ecalectin
- Rat IgG2a, κ
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- Product Citations
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Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc); Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts and thymus epithelial cells. It may be retained intracellularly or be transported to the cell surface where it is cleaved, thereby generating a soluble form. Galectin-9 is involved in events such as cell aggregation, cell adhesion, chemotaxis and apoptosis. Importantly for the regulation of the immune response, Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.Product Details
- Antibody Type
- Host Species
- Full length recombinant mouse Galectin-9 (M-type)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
IHC-F - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Clone 108A2 recognizes the linker peptide of mouse Galectin-9; it does not cross-react with stable Galectin-9 (which is biologically active, genetically engineered, linker-less Galectin-9)3. Additional reported applications (for the relevant formats) include: sandwich ELISA as capture antibody1 and immunohistochemical staining of paraffin2 embedded tissue sections.
(PubMed link indicates BioLegend citation)
AB_2810406 (BioLegend Cat. No. 137909)
AB_2810407 (BioLegend Cat. No. 137910)
- Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells
- Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
- Cell Type
- Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
- Molecular Family
- Adhesion Molecules, Immune Checkpoint Receptors
- Antigen References
1. Klibi J, et al. 2009. Blood 113:1957.
2. Seki M, et al. 2008. Clin. Immunol. 127:78.
3. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
4. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.
- Gene ID
- 16859 View all products for this Gene ID
- View information about Galectin-9 on UniProt.org
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