Alexa Fluor® 647 anti-Histone H3.3 Phospho (Ser31) Antibody

Pricing & Availability
Clone
1A8G10 (See other available formats)
Regulatory Status
RUO
Other Names
Histone-H3
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A_1A8G10_A647_Histone_H3point3_Phospho_Antibody_1_072017
HeLa cells were incubated with (top) or without (bottom) nocodozole overnight, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer then intracellularly stained with DAPI and Histone H3.3 Phospho (Ser31) (clone 1A8G10) Alexa Fluor® 647.
  • A_1A8G10_A647_Histone_H3point3_Phospho_Antibody_1_072017
    HeLa cells were incubated with (top) or without (bottom) nocodozole overnight, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer then intracellularly stained with DAPI and Histone H3.3 Phospho (Ser31) (clone 1A8G10) Alexa Fluor® 647.
  • B_1A8G10_A647_Histone_H3point3_Phospho_Antibody_1_072017
See Alexa Fluor® 647 spectral data See additional ICFC data...
Cat # Size Price Quantity Check Availability Save
687703 25 tests £120.00
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687704 100 tests £263.00
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Description

Histone 3, also known as H3, is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability. H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins. H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis. Phosphorylation of H3.3 Ser31 occurs during mitosis and is a chromatin mark associated with heterochromatin. This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Histone H3.3 S31ph peptide (24-37)(AARKSAP(phS)TGGVKK), Freund’s complete adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 1A8G10 detects histone H3.3 when phosphorylated at Ser31.

RRID
AB_2629552 (BioLegend Cat. No. 687703)
AB_2629553 (BioLegend Cat. No. 687704)

Antigen Details

Structure
H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution

Nucleus.

Function
H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability.
Interaction
Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family
Phospho-Proteins
Antigen References

1. Chang FT, et al. 2015. Nucleic Acids Res. 43:2603.
2. Sharma K, et al. 2014. Cell Rep. 8:1583.
3. Yoshimi T, et al. 2013. Monoclon. Antib. Immunodiagn. Immunother. 32:119.

Gene ID
3020 View all products for this Gene ID
UniProt
View information about Histone H3.3 Phospho Ser31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 07/20/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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