Alexa Fluor® 594 anti-Histone H3 Acetylated Lysine 9 (K9ac) Antibody

Pricing & Availability
Clone
2G1F9 (See other available formats)
Isotype
Rat IgG2a, κ
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Product Citations
publications
2G1F9_A594_Histone_H3_Acetylated_101019.png
Untreated (panel A) and sodium butyrate-treated (5 mM for 16 hours, panel B) HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 2.5 µg/mL (1:100 dilution) of Alexa Fluor® 594 anti-Histone H3 Acetylated Lysine 9 (K9ac) (clone 2G1F9) overnight at 4°C. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
  • 2G1F9_A594_Histone_H3_Acetylated_101019.png
    Untreated (panel A) and sodium butyrate-treated (5 mM for 16 hours, panel B) HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 2.5 µg/mL (1:100 dilution) of Alexa Fluor® 594 anti-Histone H3 Acetylated Lysine 9 (K9ac) (clone 2G1F9) overnight at 4°C. Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
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698403 25 µg £77
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Description

Histone subunit H3, along with subunits 2A, 2B, and 4, make up the core subunits of the nucleosome octomer. An octomer contains two protomers of each subunit tightly wrapped around a ~147 bp segment of DNA. Histones have integral roles in chromatin integrity, genomic stability, and gene regulation. Post-translational modification of histones in response to certain stimuli results in alterations of nucleosomal positioning relative to DNA.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Histone H3 K9ac peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1 - 5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Antigen Details

Structure
Histone H3 is a 134 aa protein with a predicted molecular weight of 15.4 kD. The subunit is comprised of a histone fold, an αN-helix main globular domain and a large N-terminal tail harboring residues subject to multiple types of post-translational
Distribution

Nucleus

Function
Histone acetylation is generally associated with activation of gene expression, nucleosomal redistribution, and histone deposition. One consequence of this modification is the alteration of chromatin structure to facilitate recruitment of transcription factors. Additionally, acetyl-lysine modification is also used as binding site for proteins containing bromodomains, resulting in their recruitment to the modified histones.
Interaction
Histones 2A, 2B, 4, HDAC
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Zhang, et al. 2017. Nat. Commun. 8: 14799.
2. Kanda M, et al. 2016. Proc. Natl. Acad. Sci. USA 113: E3394.
3. Bellet MM, et al. 2013. Proc. Natl. Acad. Sci. USA 9: 3333.
4. Li B, et al. 2016. Sci. Rep. 6: 26542.
5. Li D, et al. 2014, Mol. Cancer. 13:26.

Gene ID
NA
UniProt
View information about Histone H3 Acetylated Lysine 9 on UniProt.org

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Go To Top Version: 1    Revision Date: 10/10/2019

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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