Alexa Fluor® 488 anti-rat IgM Antibody

Pricing & Availability
Clone
MRM-47 (See other available formats)
Other Names
Immunoglobulin M
Isotype
Mouse IgG
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MRM-47_A647_IgM_Antibody_1_062819
LEW/s rat splenocytes were stained with rat IgM(clone MRM-47) Alexa Fluor® 488(filled histogram), or rat IgM isotype control(open histogram).
  • MRM-47_A647_IgM_Antibody_1_062819
    LEW/s rat splenocytes were stained with rat IgM(clone MRM-47) Alexa Fluor® 488(filled histogram), or rat IgM isotype control(open histogram).
  • MRM-47_A647_IgM_Antibody_2_062819
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Purified PNAd (clone MECA-79) at 4°C overnight. The staining was followed by 2.5 µg/ml anti-rat IgM (MRM-47) Alexa Fluor® 488 (green) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594(red) at room temperature for 2 hours. The image was captured by 10X objective.
  • MRM-47_A647_IgM_Antibody_3_062819
    C57BL/6 mouse frozen lymph node section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Purified PNAd (clone MECA-79) at 4°C overnight. The staining was followed by 2.5 µg/ml anti-rat IgM (MRM-47) Alexa Fluor® 488 (green) as a secondary, and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 594(red) at room temperature for 2 hours. The image was captured by 10X objective.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
408907 25 µg £57
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408908 100 µg £131
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Description

The MRM-47 monoclonal antibody reacts with rat immunoglobulin M (IgM) in all tested rat strains (Lou, Lou/Ws1/M, Lewis, Wistar, DA, Sprague-Dawley). It does not react with other isotypes. The MRM-47 antibody can be used as a primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mixed rat Igs
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® is a trademark of Life Technologies Corporation.

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Go To Top Version: 1    Revision Date: 06/28/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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