So you have a new CyTOF® instrument? Then get CyTOF®-validated purified antibodies for metal labeling from BioLegend. Maxpar® Ready purified antibodies are optimized and ready-to-use with Maxpar® metal labeling kits from Fluidigm. Why choose Maxpar® Ready purified antibodies?

  • High concentration (1.0 mg/ml), optimal for conjugation
  • No additional proteins in the buffer
  • No buffer exchanges required, ready-to-use
  • Ultra-low protein binding tubes guarantees accuracy of antibody amount
  • High quality, low cost

CyTOF® mass cytometry is an emerging and exciting application for research

CyTOF® mass cytometry utilizes rare metals tagged to antibodies, allowing researchers to examine over 30 different proteins on and within a single cell without concern of overlapping emission spectra. This single cell proteomics platform enables simultaneous deep phenotyping with functional profiling and has already been used in a diverse range of research areas, including immunology, oncology and hematopoietic stem cell research.

How does it work?

Cells suspensions are stained with a panel of varying metal-conjugated probes (typically antibodies) directed against specific targets of interest. The cells are then introduced into an Inductively Coupled Plasma where they are atomized and the cell-associated metals are ionized. The atomic ions for each single cell is extracted into the ion optics and time-of-flight regions of the mass cytometer where the ions are separated by mass. The masses corresponding to the metal-tagged probes are counted in discrete time-separated detector channels, allowing for quantitation of each target of interest per cell.

The mass cytometer allows for exceptionally high speed data acquisition, with four minutes of raw data collection being sufficient for analysis of 100,000 cells. The resulting data is written in .txt or .fcs file formats compatible with many analysis programs.

The CyTOF® mass cytometry platform truly unlocks the ability to comprehensively profile and phenotype complex samples.

image 1

SPADE diagram of PBMC’s analyzed by CyTOF® mass cytometry.  Node size represents the number of cells and the color indicates the expression of CD4.

image 2

CyTOF 2: Time-of-Flight mass spectrometer for high-speed acquisition of highly multi-parametric single cell data. 

Optimized for Maxpar® Metal Labeling Kits

BioLegend offers a large selection of CyTOF®-validated Maxpar® Ready purified antibodies for human and mouse research. View the CyTOF® data on each datasheet.

Listen to the LEGENDeers latest release: Heavy Metal Cytometry.

 

 

 

Download the song now at cdbaby.com

 

Video coming soon…

 

Heavy Metal Cytometry Lyrics

 

Oh Antibody, find me sweet cytometry

 

I don’t need no fluorescence

No need to compensate

Just need some lessons

on making metal conjugates

 

Forty antibody panel

Can’t even contemplate

Gonna need forty channels

To get my data straight

 

I’m looking for Heavy Metal Cytometry

I really need Heavy Metal Cytometry

Gonna get more Heavy Metal Cytometry

Got my PhD in Heavy Metal Cytometry

 

I don’t need no buffers

I’ve got time of flight

Don’t need to suffer

To get my phenotype

 

Don’t need no laser lights

I’ve got my isotopes

To find my lymphocytes

Pushing the envelope

 

Chorus

 

Gotta set my cells on fire

And watch the data get higher

 

Chorus

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