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- Clone
- W19171A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Recombinant human IFN-γ (Cat. No. 570206) (black circles) decreased the cell viability of HT-29 cells with recombinant human TNF-α (Cat. No. 570106) at 0.1 µg/mL. Ultra-LEAF™ purified anti-human IFN-γ (clone W19171A) (purple squares) reverses the effect of recombinant human IFN-γ at 0.08 µg/mL and recombinant human TNF-α at 0.1 µg/mL on HT-29 cells in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have the effect. ND50 range : 1 - 5 μg/mL
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 611853 | 100 µg | 287€ | ||||
| 611854 | 1 mg | 964€ | ||||
Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ demonstrates anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human IFN-γ
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
Neut - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by inhibiting the cytotoxity induced by recombinant human IFN-γ (Cat. No. 570206) at 0.08 μg/mL and recombinant human TNF-α (Cat. No. 570106) at 0.1 μg/mL on HT-29 cells. ND50 range: 1.0 - 5.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2941603 (BioLegend Cat. No. 611853)
AB_2941603 (BioLegend Cat. No. 611854)
Antigen Details
- Structure
- Cytokine, dimer
- Distribution
-
CD8+ and CD4+ T cells, NK cells
- Function
- Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
- Interaction
- T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
- Ligand/Receptor
- IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
- Cell Type
- NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Stem Cells
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
- Fitzgerald K, et al. 2001. The Cytokine FactsBook Academic Press San Diego.
- De Maeyer E, et al. 1992. Curr Opin Immunol. 4:321-6.
- Farrar M, et al. 1993. Annu Rev Immunol. 11:571-611.
- Gray P, et al. 1987. Lymphokines. 13:151.
- Gene ID
- 3458 View all products for this Gene ID
- UniProt
- View information about IFN-gamma on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All IFN-γ Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Ultra-LEAF™ Purified anti-human IFN-γ | W19171A | Neut |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Ultra-LEAF™ Purified anti-human IFN-γ
Recombinant human IFN-γ (Cat. No. 570206) (black circles) de...
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