FITC anti-rat CD4 (domain 2) Antibody

Pricing & Availability
Clone
OX-35 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Mouse IgG2a, κ
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Product Citations
publications
OX-35_FITC_011909.jpg
LOU rat splenocytes stained with OX-35 FITC
  • OX-35_FITC_011909.jpg
    LOU rat splenocytes stained with OX-35 FITC
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203305 100 µg 123€
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Description

CD4, also known as T4, is a 55kD glycoprotein member of the immunoglobin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MLR generated rat T helper lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The OX-35 antibody has been shown to bind a different CD4 epitope than the W3/25 and OX-38 antibodies. Additional reported applications (for the relevant formats) include: immunohistochemical staining2.

Application References

(PubMed link indicates BioLegend citation)
  1. May E, et al. 2003. J. Immunol. 170:1099.
  2. Hansson AS, et al. 1999. J. Clin. Invest. 104:589. (IHC)
Product Citations
  1. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
RRID
AB_1501180 (BioLegend Cat. No. 203305)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution
Majority of thymocytes, T cell subset
Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Thymocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. May E, et al. 2003. J. Immunol. 170:1099.
2. Hansson AS, et al. 1999. J Clin Invest. 104:589

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08.22.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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