Loading...

Biotin anti-human TCR α/β Antibody

Pricing & Availability
Clone
IP26 (See other available formats)
Regulatory Status
RUO
Other Names
α/β TCR, TCR α/β
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
IP26_Biotin_072607
Human peripheral blood lymphocytes stained with biotinylated IP26, followed by Sav-PE
  • IP26_Biotin_072607
    Human peripheral blood lymphocytes stained with biotinylated IP26, followed by Sav-PE
Compare all formats
Cat # Size Price Quantity Check Availability Save
306703 25 µg 67€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
306704 100 µg 151€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone.  Other anti-CD3 clones may compete out the binding of IP26.  

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC)
  2. Joseph A, et al. 2008. J. Virol. 82:3078. (FC) PubMed
  3. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
Product Citations
  1. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  2. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
  3. Zah E, et al. 2020. Nat Commun. 11:2283. PubMed
  4. Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed
  5. Bear AS, et al. 2021. Nat Commun. 12:4365. PubMed
RRID
AB_314629 (BioLegend Cat. No. 306703)
AB_314632 (BioLegend Cat. No. 306704)

Antigen Details

Structure
Ig superfamily, with CD3 forms CD3/TCR complex
Distribution

T cells, thymocytes

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound to MHC
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.

Gene ID
6955 View all products for this Gene ID 6957 View all products for this Gene ID
UniProt
View information about TCR alpha/beta on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human TCR α/β

  • FITC anti-human TCR α/β

  • PE anti-human α/β T Cell Receptor

  • PE/Cyanine5 anti-human TCR α/β

  • Purified anti-human TCR α/β

  • PE/Cyanine7 anti-human TCR α/β

  • Alexa Fluor® 488 anti-human TCR α/β

  • Alexa Fluor® 647 anti-human TCR α/β

  • Pacific Blue™ anti-human TCR α/β

  • APC anti-human TCR α/β

  • Brilliant Violet 421™ anti-human TCR α/β

  • PerCP/Cyanine5.5 anti-human TCR α/β

  • PE/Dazzle™ 594 anti-human TCR α/β

  • APC/Cyanine7 anti-human TCR α/β

  • Alexa Fluor® 700 anti-human TCR α/β

  • Brilliant Violet 510™ anti-human TCR α/β

  • APC/Fire™ 750 anti-human TCR α/β

  • Brilliant Violet 605™ anti-human TCR α/β

  • TotalSeq™-A0224 anti-human TCR α/β

  • Brilliant Violet 711™ anti-human TCR α/β

  • Brilliant Violet 785™ anti-human TCR α/β

  • TotalSeq™-C0224 anti-human TCR α/β

  • Brilliant Violet 750™ anti-human TCR α/β

  • TotalSeq™-B0224 anti-human TCR α/β

  • PE anti-human TCR α/β

  • APC/Fire™ 810 anti-human TCR α/β

  • TotalSeq™-D0224 anti-human TCR α/β

  • GMP PE anti-human TCR α/β
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account