APC anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
OKT4_APC_060507
Human peripheral blood lymphocytes stained with OKT4 APC
  • OKT4_APC_060507
    Human peripheral blood lymphocytes stained with OKT4 APC
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317415 25 tests 27€
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317416 100 tests 58€
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
  1. Carre C, et al. 2021. iScience. 24:102970. PubMed
  2. Wang T, et al. 2022. Front Immunol. 13:954121. PubMed
  3. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  4. Claiborne DT, et al. 2019. PLoS Pathog. 15:e1007981. PubMed
  5. Mete B, et al. 2022. Life Sci Alliance. 5:. PubMed
  6. Uhlenbrock F, et al. 2014. J Immunol. 193:1654. PubMed
  7. Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
  8. Want MY, et al. 2019. Oncoimmunology. 8:e1586042. PubMed
  9. Sun J, et al. 2018. Scand J Immunol. 88:e12674. PubMed
  10. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  11. Saadati S, et al. 2020. Avicenna J Med Biotechnol. 0.591666667. PubMed
  12. Zhu ZY, et al. 2022. Front Immunol. 12:781087. PubMed
  13. Dushyanthen S, et al. 2017. Nat Commun. 10.1038/s41467-017-00728-9. PubMed
  14. Wang Z, et al. 2021. Cell Mol Immunol. 18:2188. PubMed
  15. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  16. Altman K 2006. J Immunol . 177:1721. PubMed
  17. Li N, et al. 2021. STAR Protoc. 2:100942. PubMed
  18. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  19. Acerbi E, et al. 2016. Sci Rep. 6:23128. PubMed
  20. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  21. Arif S, et al. 2020. Diabetologia. 63(6):1186-1198.. PubMed
  22. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  23. Jiang H, et al. 2021. Oncoimmunology. 10:1943180. PubMed
  24. Narsale A, Moya R, Robertson H 2016. Data Brief. 8: 1348-51. PubMed
  25. Sugita S, et al. 2016. Stem Cell Reports. 7:619-634. PubMed
  26. Wang A, et al. 2020. Front Immunol. 1.213194444. PubMed
  27. Zhang J, et al. 2022. Nature. 609:369. PubMed
  28. Berezhnoy A, et al. 2020. Cell Rep Med. 1:100163. PubMed
  29. Johnson A, et al. 2016. mSphere. 1: e00288-16. PubMed
  30. Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed
  31. Kim K, et al. 2019. Nat Microbiol. 1.586111111. PubMed
  32. Jafari M, et al. 2014. J Virol. 88:5062. PubMed
  33. Frencher J, et al. 2014. J Leukoc Biol. 96:957. PubMed
  34. Li N, et al. 2021. Cell Rep Med. 2:100297. PubMed
  35. Dahal S, et al. 2022. Retrovirology. 19:18. PubMed
  36. Li N, et al. 2020. Oncoimmunology. 9:1824643. PubMed
  37. Bossini-Castillo L, et al. 2022. Cell Genom. 2:. PubMed
  38. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  39. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  40. Ledderose C, et al. 2021. J Leukoc Biol. 109:497. PubMed
  41. Dupont L, et al. 2021. Cell Host Microbe. 29(5):792-805.e6. PubMed
  42. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  43. Comte D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9321 - 9326. PubMed
  44. Mujib S, et al. 2017. JCI Insight. 2:e93687. PubMed
  45. Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed
  46. Su Y, et al. 2020. Cell. 1479:183. PubMed
  47. Sashihara J, et al. 2011. PLoS One. 6:e1002308. PubMed
  48. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  49. Siedlik J, et al. 2017. J Immunol Methods. 10.1016/j.jim.2017.03.017. PubMed
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  52. Dotson A, et al. 2013. Clin Immunol. 148:149. PubMed
RRID
AB_571944 (BioLegend Cat. No. 317415)
AB_571945 (BioLegend Cat. No. 317416)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 4    Revision Date: 07.13.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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