APC anti-human CD184 (CXCR4) Antibody

Pricing & Availability
Clone
12G5 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70204
Other Names
CXCR4, Fusin
Isotype
Mouse IgG2a, κ
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Product Citations
publications
12G5_APC_071607
Human peripheral blood lymphocytes stained with 12G5 APC
  • 12G5_APC_071607
    Human peripheral blood lymphocytes stained with 12G5 APC
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306509 25 tests 124€
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306510 100 tests 260€
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Description

CD184, also known as fusin or CXCR4, is a 45 kD seven transmembrane G-protein-linked CXC chemokine receptor. CD184 is widely expressed on blood and tissue cells, including B and T cells, monocytes, macrophages, dendritic cells, granulocytes, megakaryocytes/platelets, lymphoid, myeloid precursor cells, endothelial cells, epithelial cells, astrocytes, and neurons, among other tissue cells. CD184 is the receptor for CXC chemokine SDF-1, mediates blood cell migration, and is involved in B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, and cerebellar development. CXCR4 is also a coreceptor of X4 HIV-1 and an alternative receptor for some isolates of HIV-2.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon, Chimpanzee, Sooty Mangabey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CP-MAC-infected Sup-T1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections11, immunocytochemistry3, immunofluorescence microscopy2,6, and blocking of CD4-independent infection by HIV-2 and CD4-dependent infection by some T cell-tropic isolates of HIV-14,5. Clone 12G5 may not be suitable for Western blotting.10 The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 306539 & 306540).

Application References

(PubMed link indicates BioLegend citation)
  1. McKnight A, et al. 1997. J. Virol. 71:1692.
  2. Endres MJ, et al. 1996. Cell 87:745. (Immunogen, IF)
  3. Volin MV, et al. 1998. Biochem. Biophys. Res. Commun. 242:46. (ICC)
  4. Berndt C, et al. 1998. P. Natl. Acad. Sci. USA 95:12556. (Block)
  5. Ullrich CK, et al. 2000. Blood 96:1438. (Block)
  6. Murga M, et al. 2005. Blood 105:1992. (IF)
  7. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC) PubMed
  8. Isnardi I, et al. 2010. Blood 115:5026. PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  10. Fischer T, et al. 2008. PLoS One 3:e4069.
  11. Schmid BC, et al. 2004. Breast Cancer Res. Treat. 84:247. (IHC)
Product Citations
  1. Gao Y, et al. 2016. Nat Methods. 10.1038/nmeth.4042. PubMed
  2. Roco JA et al. 2019. Immunity. 51(2):337-350 . PubMed
  3. Biasci D, et al. 2020. Proc Natl Acad Sci U S A. 117:28960. PubMed
  4. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  5. Xu X, et al. 2021. Molecular Cell. :. PubMed
  6. Isnardi I, et al. 2010. Blood. 115:5026. PubMed
  7. Gludish DW, et al. 2020. Sci Rep. 10:19900. PubMed
  8. Jayasinghe MK, et al. 2022. Theranostics. 12:3288. PubMed
  9. McQuade A, et al. 2020. Nat Commun. 4.1875. PubMed
  10. Agha O, et al. 2020. JOR Spine. 3:e1091. PubMed
  11. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  12. Roukens AHE, et al. 2022. Nat Immunol. 23:23. PubMed
  13. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
  14. Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed
  15. Hyun Hee Lee, Vanessa Bellat, Benedict Law 2017. PLoS One. 12(2):e0171044. PubMed
  16. Huijbregts R, et al. 2013. Endocrinology. 154:1282. PubMed
  17. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  18. Cai J, et al. 2021. eLife. 10:00. PubMed
  19. Fiedorowicz M, et al. 2020. Sci Rep. 10:5379. PubMed
  20. Rai MA, et al. 2020. Retrovirology. 17:35. PubMed
  21. Kempton HR, et al. 2020. Mol Cell. 184:78. PubMed
  22. Foight GW, et al. 2019. Nat Biotechnol. 37:1209. PubMed
  23. Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed
  24. Suzuki S, et al. 2021. STAR Protocols. 2:100683. PubMed
  25. Toyoda M, et al. 2015. J Virol. 89: 9639-9652. PubMed
  26. Shi J, et al. 2012. Blood. 120:1624. PubMed
  27. Meng YM, et al. 2018. Oncoimmunology. 7:e1408745. PubMed
  28. Walker KL, et al. 2021. Leuk Lymphoma. 62:1167. PubMed
  29. Grosche L, et al. 2020. Viruses. 12:. PubMed
  30. Blanco-Camarillo C, et al. 2021. Front Immunol. 12:672520. PubMed
  31. Nakamura M, et al. 2019. Nat Commun. 10:194. PubMed
  32. Ogaki S, et al. 2015. Sci Rep. 5:17297. PubMed
  33. Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed
  34. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
  35. Shan L, et al. 2017. Immunity. 47:766. PubMed
  36. Rodrigues Toste de Carvalho AL, et al. 2021. Nat Protoc. 16:1802. PubMed
  37. Olety B, et al. 2021. Sci Adv. 7:eabj7398. PubMed
  38. Samuel RM, et al. 2020. Cell Stem Cell. 27:876. PubMed
RRID
AB_314615 (BioLegend Cat. No. 306509)
AB_314616 (BioLegend Cat. No. 306510)

Antigen Details

Structure
Rhodopsin family, G-protein linked seven transmembrane glycoprotein, 45 kD
Distribution

T cells and B cells, dendritic cells, monocytes, granulocytes, hematopoietic progenitors, endothelial cells

Function
B lymphopoiesis and myelopoiesis, cardiogenesis, blood vessel formation, cerebellar development
Ligand/Receptor
SDF-1 receptor, coreceptor for X4 HIV-1
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Monocytes, Neural Stem Cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Berger E, et al. 1999. Annu. Rev. Immunol. 17:657.
2. Loetscher P, et al. 2000. Adv. Immunol. 74:127.
3. Murphy P, et al. 2000. Pharmacol. Rev. 52:145.

Gene ID
7852 View all products for this Gene ID
UniProt
View information about CD184 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 4    Revision Date: 08.22.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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