7-AAD Viability Staining Solution

Pricing & Availability
Regulatory Status
RUO
Other Names
Viability dye, dead cell exclusion dye, cell viability dye
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Product Citations
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420403 200 tests 23€
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420404 500 tests 44€
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Description

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Product Details
Technical data sheet

Product Details

Formulation
PBS, pH 7.2 with 0.09% (w/v) sodium azide.
Preparation
CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Concentration
50 µg/ml
Storage & Handling
Protect from light. Store between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 µl of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. The analysis of the cells should be done as soon as possible after the incubation with the dye.

We recommend utilizing our Fluorescence Spectra Analyzer to determine compatibility of 7AAD with other fluorochromes.

 

Excitation Laser
Blue Laser (488 nm)
Application Notes

7-AAD Viability Staining Solution can be used as a viability probe for methods of nonviable cell exclusion.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Paterson AM, et al. 2011. J. Immunol. 187:1097. PubMed
  2. Wingren M, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2000. PubMed
  3. Sandu SK, et al. 2012. PNAS 109:20047. PubMed
  4. Evdokimova V, et al. 2012. Sci Signal. 5:92. PubMed
  5. Choudhury KR, et al. 2012. PLoS One. 7:e51415. PubMed
  6. Wu Y, et al. 2013. J. Immunol. 190:1859. PubMed
  7. Harker KS, et al. 2013. J. Leukoc Biol. 93:789. PubMed
  8. Zhang ZN, et al. 2013. Clin Chem. 59:1175. PubMed
  9. Mikhak Z, et al. 2013. J Exp Med. 210:1855. PubMed
  10. Cohen HB, et al. 2013. Blood. 122:1935. PubMed
  11. Hufbauer M, et al. 2013. J. Virol. 87:12158. PubMed
  12. Turner JE, et al. 2103. J Exp Med. PubMed
  13. Teng T, et al. 2013. Mol Cell Biol. 33:4660. PubMed
  14. Hussain MS, et al. 2013. Hum Mol Genet. 22:5199. PubMed
  15. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
  16. Kong W, et al. 2014. Cell Immunol. 287:1. PubMed
  17. Fell LH, et al. 2014. Nephrol Dial Transplant. PubMed
  18. Kraaji MD, et al. 2014. Cytokine. 67:7. PubMed
  19. Schneider C, et al. 2014. PNAS. 111:8185. PubMed
  20. Ermann J, et al. 2014. PNAS. 111:2559. PubMed
  21. Cao L, et al. 2014. J Virol. 88:8656. PubMed
  22. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
  23. Wedel J, et al. 2014. J Leukoc Biol. 96:453. PubMed
  24. Jaworska J, et al. 2015. J Immunol. 194:325. PubMed
Product Citations
  1. Hofmann M, et al. 2012. Leukemia. 26:1288. PubMed
  2. Wigren M, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2000 . PubMed
  3. Sandhu S, et al. 2012. Proc Natl Acad Sci U S A. 109:20047. PubMed
  4. Evdokimova V, et al. 2012. Sci Signal. 5:92. PubMed
  5. Choudhury K, et al. 2012. PLoS One. 7:e51415. PubMed
  6. Wu Y, et al. 2013. J Immunol. 190:1859. PubMed
  7. Harker K, et al. 2013. J Leukoc Biol. 93:789. PubMed
  8. Zhang Z, et al. 2013. Clin Chem. 59:1175. PubMed
  9. Hussain M, et al. 2013. Hum Mol Genet. 22:5199. PubMed
  10. Mikhak Z, et al. 2013. J Exp Med. 210:1855. PubMed
  11. Hussain M, et al. 2013. Hum Mol Genet. 122:1935. PubMed
  12. Hufbauer M, et al. 2013. J Virol. 87:12158. PubMed
  13. Teng T, et al. 2013. Mol Cell Biol. 33:4660. PubMed
  14. Turner J, et al. 2013. J Exp Med. 210:2951. PubMed
  15. Kong W, et al. 2014. Cell Immunol. 287:1. PubMed
  16. Fell L, et al. 2014. Nephrol Dial Transplant. 29:809. PubMed
  17. Kraaij M, et al. 2014. Cytokine. 67:7. PubMed
  18. Schneider C, et al. 2014. Proc Natl Acad Sci U S A. 111:8185. PubMed
  19. Ermann J, et al. 2014. Proc Natl Acad Sci U S A. 111:2559. PubMed
  20. Cao L, et al. 2014. J Virol. 88:8656. PubMed
  21. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  22. Wedel J, et al. 2014. J Leukoc Biol. 96:453. PubMed
  23. Jaworska K, et al. 2015. J Immunol. 194:325. PubMed
  24. Mahiti M, et al. 2015. Biochem Biophys Res Commun. 463: Pags 248-254. PubMed
  25. Bonaccorsi-Riani E, et al. 2015. PLoS One. 10: 0136106. PubMed
  26. Lobb I, et al. 2015. J Urol. In Press, Uncorrected Proof,. PubMed
  27. Schmidt J, et al. 2015. J Immunol Methods. 423: 12-17. PubMed
  28. Otsubo D, et al. 2015. Anticancer Res. 35: 4425-4431. PubMed
  29. Guan H, et al. 2015. Oncogenesis. 4: 166. PubMed
  30. Dokun A, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 790-803. PubMed
  31. Walz S, et al. 2015. Blood. 126: 1203-1213. PubMed
  32. Paterson A, et al. 2015. J Exp Med. 212: 1603-1621. PubMed
  33. Hilliard K, et al. 2015. Infect Immun . 83: 4015 - 4027. PubMed
  34. Urbanska K, et al. 2015. Cancer Immunol Res. 3: 1130 - 1137. PubMed
  35. Leung T, et al. 2015. Genes Dev. 29: 2097 - 2107.. PubMed
  36. Kamimura D, et al. 2015. Int Immunol. 10.1093/intimm/dxv059. PubMed
  37. Campana R, et al. 2015. J Allergy Clin Immunol. pii: S0091-6749(15)01331-7. PubMed
  38. Suarez S, et al. 2015. J Biol Chem. 290: 28311 - 28320. PubMed
  39. Kumai T, et al. 2015. Sci Rep. 5:16280. PubMed
  40. Gebhardt C, et al. 2015. Clin Cancer Res. 21: 5453 - 5459. PubMed
  41. Fuchs R, et al. 2015. Toxicology. 338: 17-29. PubMed
  42. Lee Y, et al. 2016. Am J Physiol Regul Integr Comp Physiol. 310: R55 - R65. PubMed
  43. Ligtenberg M, et al. 2016. J Immunol. 196: 759 - 766. PubMed
  44. Muroski M, et al. 2016. PLoS One. 11: 0145129. PubMed
  45. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
  46. Walachowski S, et al. 2016. PLoS One. 11: 0148464. PubMed
  47. Han W, et al. 2015. Stem Cell Dev. 24: 2536-2546. PubMed
  48. Dingjan I, et al. 2016. Sci Rep. 6:22064. PubMed
  49. Catarinella M, et al. 2016. EMBO Mol Med. 8: 155 - 170. PubMed
  50. Lindgren I, et al. 2016. Hum Reprod. 31: 672 - 683. PubMed
  51. Qi X, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.25. PubMed
  52. Alikhan M, et al. 2016. J Am Soc Nephrol. 27: 706 - 714. PubMed
  53. Eipper S, et al. 2016. PLoS One. 11: 0151509. PubMed
  54. Baker A, et al. 2016. Cancer Res . 76: 1158 - 1169. PubMed
  55. Bosma M, et al. 2016. Nat Commun. 7: 11314. PubMed
  56. Peiris T, et al. 2016. Development. 143: 1697 - 1709.. PubMed
  57. Kadowaki A, et al. 2016. Nat Commun. 7:11639. PubMed
  58. Marsh T, et al. 2016. Cancer Res . 76: 2932 - 2943. PubMed
  59. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  60. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  61. Quantius J, et al. 2016. PLoS One. 12: 1005544. PubMed
  62. Palm A, Friedrich H, Kleinau S 2016. Sci Rep. 6:27687. PubMed
  63. Ihara S, et al. 2016. J Immunol. 196: 4603 - 4613. PubMed
  64. Urso K, et al. 2016. PLoS One. 11: 0158893. PubMed
  65. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  66. Coppo M, et al. 2016. Nat Commun. 7:12254. PubMed
  67. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  68. P, et al. 2016. Mol Ther. 24: 1602-1614. PubMed
  69. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  70. Gomez J, et al. 2016. J Immunol. 197: 2864 - 2879. PubMed
  71. Wu X, et al. 2016. Proc Natl Acad Sci U S A. 113(51):14775-14780. PubMed
  72. Perales-Puchalt A, et al. 2017. Clin Cancer Res. 23(2):441-453. PubMed
  73. Chimote A, et al. 2017. Cancer Res . 77(1):53-61. PubMed
  74. Kitazawa M, et al. 2017. PLoS One. 10.1371/journal.pone.0169340. PubMed
  75. Liu S, et al. 2017. PLoS One. 10.1371/journal.pone.0169701. PubMed
  76. McCaw L, et al. 2017. EBioMedicine. 15:24-35. PubMed
  77. Nkwouano V, et al. 2017. PLoS One. 12(2):e0171817. PubMed
  78. Malara A, et al. 2017. J Biol Chem. 292(8):3239-3251. PubMed
  79. Mahauad-Fernandez W, Okeoma C 2017. Cell Death Dis. 10.1038/cddis.2017.68. PubMed
  80. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  81. Parayath NN, et al. 2020. Nat Commun. 4.680555556. PubMed
  82. Glass DR, et al. 2020. Immunity. 53:217. PubMed
  83. Dewdney B, et al. 2020. Sci Rep. 10:16769. PubMed
  84. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  85. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  86. Kang MH, et al. 2020. Nat Commun. 3.186805556. PubMed
  87. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  88. Aregger M, et al. 2020. Nat Metab. 0.429861111. PubMed
  89. Rennier K, et al. 2020. Clin Cancer Res. 26:5019. PubMed
  90. Yan P, et al. 2020. Cell Rep. 31:107840. PubMed
  91. Abe S, et al. 2020. JCI Insight. 5:00. PubMed
  92. Ligon MM, et al. 2020. Mucosal Immunol. 1.172222222. PubMed
  93. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  94. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  95. Laughney AM, et al. 2020. Nat Med. 26:259. PubMed
  96. Vis B, et al. 2020. Proc Natl Acad Sci U S A. 117:285. PubMed
  97. Tamò L, et al. 2021. Int J Mol Sci. 22:00. PubMed
  98. Kho D, et al. 2017. PLoS One. 10.1371/journal.pone.0180267. PubMed
  99. Sun H, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.08.004. PubMed
  100. Sogkas G, et al. 2017. Inflammation. 10.1007/s10753-017-0661-y. PubMed
  101. Kurachi M, et al. 2017. Nat Protoc. 10.1038/nprot.2017.083. PubMed
  102. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
  103. Naqvi N,et al. 2017. Sci Rep.. 10.1038/s41598-017-12753-1. PubMed
  104. Golubovskaya V,et al. 2017. Cancers (Basel). . 10.3390/cancers9100139. PubMed
  105. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  106. Woolf N, et al. 2017. Oncogenesis. 10.1038/oncsis.2017.77. PubMed
  107. Jacobs R, et al. 2017. J Immunol Methods. . 10.1016/j.jim.2017.09.001. PubMed
  108. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  109. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  110. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  111. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  112. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
  113. Yoshioka T, et al. 2017. PLoS One. 12:e0174103. PubMed
  114. Wu SJ, et al. 2017. Cell Rep. 2.628472222. PubMed
  115. Feng W, et al. 2017. Nat Commun. 0.697916667. PubMed
  116. Contreras O, et al. 2018. Skelet Muscle. 8:05. PubMed
  117. Park CG, et al. 2018. Sci Transl Med. 10:eaar1916. PubMed
  118. Buus TB, et al. 2018. Blood Adv. 1.552083333. PubMed
  119. Wanrooy BJ, et al. 2018. J Neuroinflammation. 0.828472222. PubMed
  120. Cabron AS, et al. 2018. J Immunol. 201:3106. PubMed
  121. Li B, et al. 2019. Oncol Rep. 41:608. PubMed
  122. Sharif T, et al. 2018. Autophagy. 1.101388889. PubMed
  123. Kuljanin M, et al. 2018. Cell Rep. 25:2524. PubMed
  124. Rosenberger L, et al. 2019. Sci Rep. 0.835416667. PubMed
  125. Nixon AML, et al. 2019. Sci Rep. 0.959722222. PubMed
  126. Pedro L, et al. 2019. Small. 15:e1900224. PubMed
  127. Teo YV, et al. 2019. Cell Rep. 27:997. PubMed
  128. Sheng Y, et al. 2019. Cell Prolif. 52:e12611. PubMed
  129. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  130. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  131. Kulpa DA, et al. 2019. J Virol. 93:e00969-19. PubMed
  132. Duan Y, et al. 2019. Cell Death Dis. 0.948611111. PubMed
  133. Van Loenhout J, et al. 2019. Cancers (Basel). 1.567361111. PubMed
  134. Sifuentes–Dominguez LF, et al. 2019. Elife. 8:e49910. PubMed
  135. Tarumoto Y, et al. 2020. Blood. 135:56:00. PubMed
  136. Combes F, et al. 2019. Cancers (Basel). 1.675. PubMed
  137. Dobie R, et al. 2020. Cell Reports. 29(7):1832-1847.e8.. PubMed
  138. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  139. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  140. Pan J, et al. 2020. J Neuroinflammation. 17:97. PubMed
  141. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  142. Iida T, et al. 2019. Cell Mol Gastroenterol Hepatol. 9:277. PubMed
  143. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  144. Hanzawa N, et al. 2020. Sci Rep. 10:5181. PubMed
  145. Fortin J, et al. 2020. Cancer Cell. 37:308. PubMed
  146. Saika A, et al. 2020. FASEB Bioadv. 2:59. PubMed
  147. Tang Z, et al. 2019. Cell Rep. 28:1744. PubMed
  148. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  149. Ramstead AG, et al. 2020. Cell Rep. 30:2889. PubMed
  150. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  151. Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
  152. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  153. Kundu K, et al. 2019. Cancer Immunol Res. 1.069444444. PubMed
  154. Bowen ME, et al. 2019. Dev Cell. 50:212. PubMed
  155. Tomlinson JE, et al. 2020. PLoS Pathog. 16:e1008677. PubMed
  156. Paterson A, et al. 2011. J Immunol. 187:1097. PubMed
  157. Ho J, et al. 2011. PLoS One. 6:e16815. PubMed

Antigen Details

Biology Area
Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability, Neuroscience
Antigen References

1. Schmid I, et al. 1992. Cytometry. 13:204

Gene ID
NA

Related FAQs

Why is washing not recommended after the addition 7-AAD or PI addition when assessing viability?

These dyes bind in equilibrium with DNA. Therefore, external dye concentration must be maintained during analysis and the dye should not be washed out.

Go To Top Version: 2    Revision Date: 05.21.2014

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