- 8RB13 (See other available formats)
- Regulatory Status
- Other Names
- RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
NeoClone Catalog# W0023
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.Product Details
- Verified Reactivity
- E. coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio
- Antibody Type
- Host Species
- Purified recombinant E. coli RNA polymerase β subunit.
- Dried ascites
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Store lyophilized antibody at -20°C. Avoid repeat freeze/thaw cycles.
WB - Quality tested
ELISA - Verified
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. To reconstitute, add 100 µl of ddH2O to the tube containing antibody and mix to dissolve. For Western blotting, the suggested dilution is 1:1000 after reconsitution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.
Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.
(PubMed link indicates BioLegend citation)
- Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
- Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
- Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
- Product Citations
AB_2564524 (BioLegend Cat. No. 663903)
- 1252 amino acids with a predicted molecular weight of approximately 150 kD.
- Transcriptional regulator in bacteria.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
- Gene ID
- 948488 View all products for this Gene ID
- View information about RNA Polymerase beta on UniProt.org
|Anti-E. coli RNA Polymerase ImmunoAffinity Resin||8RB13||IP|
|Anti-E. coli RNA Polymerase β||8RB13||WB, ELISA, IP|
|Purified anti-E. coli RNA Polymerase β||8RB13||WB, ELISA, IP|
|Direct-Blot™ HRP anti-E. coli RNA Polymerase β||8RB13||WB|
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