PE/Dazzle™ 594 anti-mouse CD304 (Neuropilin-1) Antibody

Pricing & Availability
Clone
3E12 (See other available formats)
Regulatory Status
RUO
Other Names
NRP-1, VEGFR165 receptor
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
3E12_PEDazzle594_CD304_Antibody_1_022219.png
C57BL/6 mouse splenocytes were stained with CD4 APC and CD304 (Neuropilin-1) (clone 3E12) PE/Dazzle™ 594 (left) or Rat IgG2a, κ PE/Dazzle™ 594 isotype control (right).
  • 3E12_PEDazzle594_CD304_Antibody_1_022219.png
    C57BL/6 mouse splenocytes were stained with CD4 APC and CD304 (Neuropilin-1) (clone 3E12) PE/Dazzle™ 594 (left) or Rat IgG2a, κ PE/Dazzle™ 594 isotype control (right).
See PE/Dazzle™ 594 spectral data
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145217 25 µg 128,00€
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145218 100 µg 268,00€
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Description

CD304, also known as neuropilin-1, is a 140 kD type I transmembrane protein. Its extracellular region contains two CUB, two FV/FVIII, and one MAM domain. It is expressed by natural regulatory T cells (nTreg), a subset of invariant natural killer T cells (iNKT), endothelial cells, and neurons. Neuropilin-1 stabilizes the interaction between Tregs and dendritic cells, contributes to the recruitment of tumor-infiltrating Tregs in response to tumor-derived VEGF, and influences the process of angiogenesis and axon guidance. The ligands of CD304 are VEGF165 and semaphorin-3A.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Extracellular region of mouse CD304
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  2. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
  3. Verhagen J, et al. 2014. PLoS One. 9e:108023. (FC) PubMed
RRID
AB_2800678 (BioLegend Cat. No. 145217)
AB_2800679 (BioLegend Cat. No. 145218)

Antigen Details

Structure
Type I transmembrane protein, 140 kD, 2 complement binding domains (CUB), 2 coagulation factor V/VIII homology domains (FV/FVIII), one meprin, A5, receptor tyrosine phosphatase domain (MAM)
Distribution

Natural regulatory T cells (nTreg), subset of invariant natural killer T cells (iNKT), endothelial cells, neurons

Function
Angiogenesis, axon guidance, modulation of the immune response
Ligand/Receptor
VEGF165, semaphorin-3A
Cell Type
Dendritic cells, Endothelial cells, Neurons, NKT cells, Tregs
Biology Area
Angiogenesis, Cancer Biomarkers, Cardiovascular Biology, Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Adaptor Proteins, Adhesion Molecules, CD Molecules
Antigen References

1. Yadav M, et al. 2012. J. Exp. Med. 209:1713.
2. Weiss JM, et al. 2012. J. Exp. Med. 209:1723.
3. Hansen W, et al. 2012. J. Exp. Med. 209:2001.
4. Milpied P, et al. 2011. Blood 118:2993.

Gene ID
18186 View all products for this Gene ID
UniProt
View information about CD304 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02.21.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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