PE/Cyanine7 anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Regulatory Status
RUO
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
281-2_PECy7_CD138_Antibody_FC_1_060413
C57BL/6 mouse splenocytes were stained with CD19 APC and CD138 (clone 281-2) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom).
  • 281-2_PECy7_CD138_Antibody_FC_1_060413
    C57BL/6 mouse splenocytes were stained with CD19 APC and CD138 (clone 281-2) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom).
  • 281-2_PECy7_CD138_Antibody_FC_2_060413
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
142513 25 µg 120,00€
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142514 100 µg 260,00€
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  2. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  3. Franks SE, et al. 2019. J Immunol. 202:3381. PubMed
  4. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  5. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  6. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  7. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  8. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  9. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  10. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  11. New JS, et al. 2020. Immunity. 53(1):172-186.e6. PubMed
  12. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  13. Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
  14. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
RRID
AB_2562197 (BioLegend Cat. No. 142513)
AB_2562198 (BioLegend Cat. No. 142514)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.12.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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