Brilliant Violet 421™ anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
281-2_BV421_1_051812
C57BL/6 mouse bone marrow cells were stained with CD19 APC and CD138 (clone 281-2) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom). Data shown is from gated on lymphoid cell population.
  • 281-2_BV421_1_051812
    C57BL/6 mouse bone marrow cells were stained with CD19 APC and CD138 (clone 281-2) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom). Data shown is from gated on lymphoid cell population.
  • 281-2_BV421_2_051812
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
142507 125 µL 140€
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142523 50 µg 184€
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142508 500 µL 292€
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
µl sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For flow cytometric staining using the µl sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Maschmeyer P, et al. 2017. J Autoimmun.. 10.1016/j.jaut.2017.11.005. PubMed
  2. Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
  3. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  4. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  5. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  6. Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed
  7. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  8. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  9. Hatzi K, et al. 2019. Nat Immunol. 20:86. PubMed
  10. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  11. Ise W, et al. 2018. Immunity. 48:702. PubMed
  12. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  13. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  14. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  15. Wang YH, et al. 2019. Front Immunol. 10:1909. PubMed
  16. Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
  17. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  18. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  19. Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed
  20. Huang L, et al. 2015. J Immunol. 194:283. PubMed
  21. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  22. He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed
RRID
AB_11204257 (BioLegend Cat. No. 142507)
AB_2565621 (BioLegend Cat. No. 142523)
AB_11203544 (BioLegend Cat. No. 142508)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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