PE anti-mouse CD209a (DC-SIGN) Antibody

Pricing & Availability
Clone
MMD3 (See other available formats)
Regulatory Status
RUO
Other Names
CD209 antigen-like protein A, dendritic cell-specific ICAM-3-grabbing non-integrin, CIRE, CD209, CDSIGN
Isotype
Mouse IgG2c
Ave. Rating
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Product Citations
publications
MMD3_PE_CD209_Antibody_1_080421
C57BL/6 peritoneal macrophages were first treated with TruStain fcX™ (anti-mouse CD16/32) Antibody and True-Stain Monocyte Blocker™. Cells were then stained with PE anti-mouse CD209a (clone MMD3) (above) or PE mouse IgG2a, κ isotype control (below) and F4/80 (clone BM8) Alexa Fluor® 647. Data shown was derived from CD3/CD19/NK-1.1 negative populations.
  • MMD3_PE_CD209_Antibody_1_080421
    C57BL/6 peritoneal macrophages were first treated with TruStain fcX™ (anti-mouse CD16/32) Antibody and True-Stain Monocyte Blocker™. Cells were then stained with PE anti-mouse CD209a (clone MMD3) (above) or PE mouse IgG2a, κ isotype control (below) and F4/80 (clone BM8) Alexa Fluor® 647. Data shown was derived from CD3/CD19/NK-1.1 negative populations.
  • MMD3_PE_CD209_Antibody_3_122717
    CHO-DC-SIGN transfected (filled histogram) and CHO-K1 (dashed histogram) cell lines were stained with PE anti-mouse CD209a (clone MMD3) antibody.
See PE spectral data
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833003 25 µg 84,00€
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833004 100 µg 224,00€
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Description

CD209a, is also known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN) and CIRE. CD209a is the mouse homolog of human CD209, and is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. It plays a critical role in capturing and internalizing pathogens such as Streptococcus pneumoniae.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a purified extracellular domain of mouse CD209a (DC-SIGN)-IgG1 Fc fusion protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 μg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The antibody will bind to both C57 and Balb/C mouse tissues.

Product Citations
  1. Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
  2. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
RRID
AB_2721636 (BioLegend Cat. No. 833003)
AB_2721637 (BioLegend Cat. No. 833004)

Antigen Details

Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Cai M, et al. 2016. Clin. Exp. Immunol. 317-25.
2. Ponichtera HE, et al. 2014. J. Immunol. 4655-65.

Gene ID
170786 View all products for this Gene ID
UniProt
View information about CD209a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 08.04.2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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