Loading...

Direct-Blot™ HRP anti-c-Myc Antibody

Pricing & Availability
Clone
9E10 (See other available formats)
Regulatory Status
RUO
Other Names
myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
9E10_DirectBlotHRP_cmyc_Antibody_WB_021716
Recombinant Posi-Tag Epitope Tag Protein (1 µL, diluted 1:10) was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP c-myc (clone 9E10). Proteins were visualized using chemiluminescence detection.
  • 9E10_DirectBlotHRP_cmyc_Antibody_WB_021716
    Recombinant Posi-Tag Epitope Tag Protein (1 µL, diluted 1:10) was resolved by electrophoresis, transferred to a nitrocellulose membrane, and probed with Direct-Blot™ HRP c-myc (clone 9E10). Proteins were visualized using chemiluminescence detection.
Compare all formats
Cat # Size Price Quantity Check Availability Save
626804 25 µL 81€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
626803 100 µL 231€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
All Species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Rao H, et al. 2001. Nature. 410:955.
  2. Peters CS, et al. 2001. J Biol Chem. 276:13718.
  3. Munro S, Pelham HR. 1987. Cell. 48:899.
  4. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations
  1. Calvo IA, et al. 2021. iScience. 24:103338. PubMed
RRID
AB_2783361 (BioLegend Cat. No. 626804)
AB_2572009 (BioLegend Cat. No. 626803)

Antigen Details

Structure
Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
Distribution

Nuclear protein

Function
Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
Interaction
MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
Modification
Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
Biology Area
Cell Biology, Protein Purification, Protein Synthesis
Antigen References
  1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
  2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
  3. Battey J, et al. 1983. Cell 34:779.
  4. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 05.17.2023

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-c-Myc

  • Anti-c-Myc Tag (9E10) Affinity Gel

  • Biotin anti-c-Myc

  • Direct-Blot™ HRP anti-c-Myc

  • Alexa Fluor® 594 anti-c-Myc

  • Ultra-LEAF™ Purified anti-c-Myc

  • Alexa Fluor® 647 anti-c-Myc

  • Alexa Fluor® 488 anti-c-Myc Antibody
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account