- 9E10 (See other available formats)
- Regulatory Status
- Other Names
- myc tag, c-Myc tag, cMyc tag, Myc epitope tag
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.Product Details
- Verified Reactivity
- All Species
- Antibody Type
- Host Species
- Amino acids 408-439, C-terminal region of human c-myc
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
WB - Quality tested
IP, ICC, IHC-P - Verified
Purification - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunocytochemistry, a concentration range of 1.0 - 2.5 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
- Additional Product Notes
This antibody recognizes endogenous human c-Myc protein in IHC-P. Reactivity with endogenous c-Myc in other applications is not verified.
(PubMed link indicates BioLegend citation)
- Rao H, et al. 2001. Nature. 410:955.
- Peters CS, et al. 2001. J Biol Chem. 276:13718.
- Munro S, Pelham HR. 1987. Cell. 48:899.
- Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
- Product Citations
AB_2235686 (BioLegend Cat. No. 626801)
AB_2148451 (BioLegend Cat. No. 626802)
- Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
- Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
- MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
- Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
- Biology Area
- Cell Biology, Protein Purification, Protein Synthesis
- Antigen References
- Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
- Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
- Battey J, et al. 1983. Cell 34:779.
- Beimling P, et al. 1985. Biochemistry 24:6349.
- Gene ID
- 4609 View all products for this Gene ID
- View information about c-Myc on UniProt.org
Related Pages & Pathways
Other FormatsView All c-Myc Reagents Request Custom Conjugation
|Anti-c-Myc Tag (9E10) Affinity Gel||9E10||Purification,IP|
|Direct-Blot™ HRP anti-c-Myc||9E10||WB|
|Alexa Fluor® 594 anti-c-Myc||9E10||ICC|
|Ultra-LEAF™ Purified anti-c-Myc||9E10||WB,ELISA,ICC,IP,IHC-P,Purification|
|Alexa Fluor® 647 anti-c-Myc||9E10||ICC|
|Alexa Fluor® 488 anti-c-Myc Antibody||9E10||ICC|
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