BioLegend now offers a new Prime-Step™ Prestained Broad Range Protein Ladder for use in Western blot and protein purification. The three-color ladder can be used to identify molecular weights ranging from 6.5 to 270 kDa and contains 7 blue-stained bands, orange reference bands at 270 and 30 kDa, and a green reference band at 52 kDa. Supplied ready-to-use in sample loading buffer, the ladder offers great resolution for a wide molecular weight range, is compatible with a variety of SDS-PAGE gel and buffer systems, and has excellent transfer efficiency on both PVDF and nitrocellulose membranes.
3 μL Prime-Step™ Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems, including 14%, 4-20% and 4-12% Tris-Glycine gel, 4-12% Bis-Tris gel (MOPS and MES running buffer), 3-8% Tris-Acetate gel and 10-20% Tricine gel. Then protein ladders were transferred to nitrocellulose membrane. Mw: molecular weight.
Prime-Step™ Prestained Broad Range
Protein ladder
Size Cat No.
50 μL 773301
500 μL 773302
Specificity Clone
Direct-Blot™ HRP anti-β actin W16197A
HRP anti-Clusterin A15113A
HRP anti-Myelin CNPase SMI91
Purified anti-β-Tubulin O95C1
Purified anti-Histone H2B W17025A
Purified anti-LCK A17013D
Purified anti-Pan-Shank N23B/49
Purified anti-RUNX1 W16118A
Purified anti-STAT1 Phospho (Tyr701) A17012A
Purified anti-Tata Binding Protein 1TBP18
Purified anti-TFEB A17106C
Substrate Kit
Western-Ready™ ECL Substrate Kit
Western Blot Antibody Sampler Kits
E. coli RNA Polymerase Antibody Sampler Kit
HDAC Antibody Sampler Kit
IRF Antibody Sampler Kit
Anti c-Myc Antibodies
Anti-DYKDDDDK Tag Antibodies
Anti-GST Antibody
Anti-HA Tag Antibodies
Anti-His Tag Antibodies
Epitope Tag Small Motif Antibody Sampler Kit
Epitope Tag Big Motif Antibody Sampler Kit
Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody

HA tagged CHO cells stained with Alexa Fluor® 488 (green) anti-HA.11 (clone 16B12) epitope tag antibody (Cat. No. 901509). Cells were counterstained with DAPI (blue).

Get a 50% off discount on our Epitope Tag reagents

*Put "50% epitope tag" in the webform's "Subject" field. Smallest available sizes only. Offer valid till May 31st, 2018. Some restrictions may apply.


One way that we address reproducibility in research is by validating antibody specificity. Learn more about how we are validating our western blot antibodies using CRISPR/Cas9 and siRNA on our new Knockout and Knockdown Validation webpage.
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