E. coli RNA Polymerase Antibody Sampler Kit

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Other Names
RNA pol beta prime, rpoC, tabB, DNA-directed, RNAP subunit beta prime, Transcriptase subunit beta prime, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit alpha, Transcriptase subunit alpha, pez, phs, sez
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A_Ecoli_RNA_Polymerase_Kit_NT73_102017
Total lysates (15 µg protein) from HeLa (lane 1) and E. coli BL21 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (1 µg/mL) purified anti-E. coli RNA Polymerase β Prime antibody (clone NT73). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • A_Ecoli_RNA_Polymerase_Kit_NT73_102017
    Total lysates (15 µg protein) from HeLa (lane 1) and E. coli BL21 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:1000 diluted (1 µg/mL) purified anti-E. coli RNA Polymerase β Prime antibody (clone NT73). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • B_Ecoli_RNA_Polymerase_Kit_NT63_102017
    Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase β antibody (clone NT63). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: MW ladder.
  • C_Ecoli_RNA_Polymerase_Kit_4RA2_102017
    Total lysates (15 µg protein) from HeLa (lane 1) and E. coli BL21 (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-E. coli RNA Polymerase α antibody (clone 4RA2). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Lane M: MW ladder.
  • D_Ecoli_RNA_Polymerase_Kit_8RB13_102017
    Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase β antibody (clone 8RB13). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: MW ladder.
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699907 1 kit 220€
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Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. α subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme. The β subunit is the second-largest subunit, and is encoded by the rpoB gene. The β’ (β prime) subunit contains structures crucial for transcription, including the sites for ribonucleotide addition and catalysis.

For additional antigen details, please refer to individual product datasheets.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype MW (kD)
E. coli RNA Polymerase β Prime NT73 25 µg E. coli, Enterics, Borellia burgdorferi, Salmonella Mouse IgG1 155
E. coli RNA Polymerase β NT63 25 µg E. coli Mouse IgG2a 150
E. coli RNA Polymerase α 4RA2 25 µg E. coli, Widely among Gram-negative bacteria, some Gram-positive bacteria Mouse IgG1 36
E. coli RNA Polymerase β 8RB13 25 µg E. coli, Bordetella, Chlamydia, Myxococcus, Salmonella, Vibrio Mouse IgG1 150


* For detailed information about each specificity, please follow the provided links. 

Product Details

Formulation
Please refer to individual product datasheets for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western Blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-E. coli RNA Polymerase β Prime (clone NT73): 1:1000-1:4000 (0.25-1ug/mL)
Anti-E. coli RNA Polymerase β (clone NT63): 1:2500-1:100000 (0.005-0.2ug/mL)
Anti-E. coli RNA Polymerase α (clone 4RA2): 1:500-1:5000 (0.1-1.0 ug/mL)
Anti-E. coli RNA Polymerase β (clone 8RB13): 1:2500-1:100000 (0.005-0.2ug/mL)

It is recommended that the reagent be titrated for optimal performance for each application.  

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

Clone NT73 recognizes epitope 1295-1417 (SLAELLNAGLGGS), and is extremely useful for purifying holoenzymes from enterics. It does not cross-react with mycobacterial smegmatis RNAP. This antibody is used for purifying Softag 1 epitope tagged protein expressed in eukaryotic systems. 

Clone NT63 recognizes epitope 922-1090 of β subunit of the E. coli RNA polymerase and can be used in WB analysis. 

Clone 8RB13 is extremely useful for detecting (WB) or purifying (IP) core RNAP from a broad range of bacteria. 

Clone 4RA2 specifically recognizes α subunit of the E. coli RNA polymerase.

Antigen Details

Biology Area
Cell Biology
Antigen References
  1. Thompson NE, et al. 1992. Biochemistry 31:7003.
  2. Thompson NE, et al. 2003. Anal. Biochem. 323:171.
  3. Lesley SA and Burgess RR. 1989. Biochemistry 28:7728.
Gene ID
948488 View all products for this Gene ID 947794 View all products for this Gene ID

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Go To Top Version: 1    Revision Date: 10.20.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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