Ultra-LEAF™ Purified anti-human IL-3 Antibody

Pricing & Availability
Clone
A15130B (See other available formats)
Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast cell growth factor (MGF/MCGF), Multi-colony stimulating factor (multi-CSF), P cell stimulating activity (PCSA)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A15130B_ULEAF_IL-3_NEUT_Antibody_020717
Recombinant human IL-3 induces the proliferation of TF-1 cells in a dose dependent manner (black squares). Ultra-LEAF™ purified anti-human IL-3 antibody (clone A15130B, purple circles) neutralizes the TF-1 cell proliferation induced by recombinant human IL-3 (1.0 ng/mL). The ND50 = 0.3 - 1.5 µg/mL.
  • A15130B_ULEAF_IL-3_NEUT_Antibody_020717
    Recombinant human IL-3 induces the proliferation of TF-1 cells in a dose dependent manner (black squares). Ultra-LEAF™ purified anti-human IL-3 antibody (clone A15130B, purple circles) neutralizes the TF-1 cell proliferation induced by recombinant human IL-3 (1.0 ng/mL). The ND50 = 0.3 - 1.5 µg/mL.
Cat # Size Price Quantity Avail. Save
694303 100 µg 196€
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694304 1 mg 520€
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Description

Interleukin-3 (IL-3) is a hemopoietic growth factor involved in the survival, proliferation, and differentiation of multipotent hemopoietic cells. IL-3 is the most potent growth factor for basophils, followed by granulocyte-macrophage colony-stimulating factor, and IL-5. These cytokines also act on mature basophils through specific receptors, thereby mediating adhesion, migration, and releasability. IL-3 is highly expressed by mast cells, and large and rapidly released amounts of autocrine IL-3 production are responsible for mast cell survival by IgE in the absence of antigen. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including: asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis.  IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. IL-3 plays a vital role in stimulating basophils and mast cell responses to parasite infections, and since basophils play an important role in Th2 immune responses, IL-3 is a critical regulator of allergic inflammation. IL-3 is expressed in the major embryonic vessels and regulates the survival and proliferation of hematopoietic stem cells in the early stages of embryonic development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human IL-3
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by neutralizing activity. The ND50 is 0.3 – 1.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2650727 (BioLegend Cat. No. 694303)
AB_2650728 (BioLegend Cat. No. 694304)

Antigen Details

Structure
Cytokine
Distribution
Activated T cells, Th1, Th2, mast cells, eosinophils, keratinocytes, NK cells, and endothelial cells.
Function
IL-3 is a pleiotropic factor which promotes both self-renewal and differentiation of early multipotential progenitors and is involved in inducible hematopoiesis in response to infections.
Interaction
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages.
Ligand/Receptor
Heterodimer IL-3Rα (CD123) and a common β subunit (βc) that is shared with the IL-5R and GM-CSFR.
Cell Type
T cells, Th1, Th2, Mast cells, Eosinophils, NK cells, Endothelial cells
Biology Area
Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Kitamura T, et al. 1989. J. Cell. Physiol. 140:323.
2. Kohno M, et al. 2005. Blood 105:2059.
3. Murphy JM, Young IG. 2006. Vitam. Horm. 74:1.
4. Kleemann R, et al. 2008. Cardiovasc. Res. 79:360.
5. Valent P, and Dahinden CA. 2010. Curr. Opin. Hematol. 17:60.
6. Mirza S, et al. 2010. J. Biol. Chem. 285:22370.

Gene ID
3562 View all products for this Gene ID
UniProt
View information about IL-3 on UniProt.org

Related FAQs

Does BioLegend test each LEAF™ antibody by functional assay?

No, BioLegend does not test LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this LEAF™/Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 02-08-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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