Recombinant Mouse M-CSF (carrier-free)

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Product Citations
M-NFS-60 cell proliferation induced by mouse M-CSF.
  • a_M-CSF_Mouse_031512
    M-NFS-60 cell proliferation induced by mouse M-CSF.
  • b_MCSF_Mouse_RECOM_BA_020923
    Recombinant mouse M-CSF induces proliferation of M-NFS-60 cells in a dose dependent manner. BioLegend’s protein was compared side-by-side to a competitor’s equivalent product.
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576402 10 µg 81€
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576404 25 µg 123€
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576406 100 µg 400€
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576408 500 µg 1688€
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M-CSF was first characterized as a glycoprotein that induces monocyte and macrophage colony formation from precursors in murine bone marrow cultures. M-CSF binds CD14+ monocytes and promotes the survival/proliferation of peripheral blood monocytes. In addition, M-CSF enhances inducible monocyte functions including phagocytic activity, microbial killing, and cytotoxicity for tumor cells as well as induces the synthesis of inflammatory cytokines such as IL-1, TNFα, and IFNγ in monocytes.

Multiple CSF1 mRNA species have been described that arise from alternative splicing in exon 6 and the alternative use of the 3’ end of exons 9 or 10. As a result, two distinct CSF1 protein products are encoded by these transcripts: a cell-surface or membrane-bound form of CSF1 (mCSF1) and a soluble form (sCSF1). Uterine sCSF1 is highly increased during pregnancy. On the contrary, uterine mCSF1 remains low during pregnancy. High levels of M-CSF have been associated to different pathologies such as pulmonary fibrosis and atherosclerosis.

M-CSF binds to its receptor M-CSFR, and this receptor is shared by a second ligand, IL-34. Mouse M-CSF and IL-34 exhibit cross-species specificity, both bind to the human and mouse M-CSF receptors. IL-34 can regulate myeloid development and substitute for CSF-1 in vivo. IL-34 has overlapping but not identical biological activities as M-CSF.

Product Details
Technical Data Sheet (pdf)

Product Details

Mouse M-CSF, amino acids Lys33-Glu262 (Accession# NM_001113530.1) was expressed in 293E cells.
Molecular Mass
The 251 amino acid recombinant protein has a predicted molecular mass of approximately 28.2 kD. The DTT-reduced and non-reduced protein migrate at approximately 50 kD and 100 kD respectively by SDS-PAGE. The N-terminal contains a His9-(SGGG)2-IEGR-tag.
>98%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in PBS.
Endotoxin Level
Less than 0.01 ng per µg cytokine as determined by the LAL method.
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
ED50 =2 - 6 ng/ml, corresponding to a specific activity of 1.6 - 5 x 105 units/mg, as determined by M-NFS60 cell proliferation induced by mouse M-CSF in a dose dependent manner.


Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at

Product Citations
  1. Jiang Z, et al. 2021. J Clin Invest. 131: . PubMed
  2. Miki S, et al. 2021. Sci Rep. 11:22469. PubMed
  3. Yokozeki Y, et al. 2021. Biomed Res Int. 2021:7988320. PubMed
  4. Kuo PC, et al. 2021. Brain Commun. 3:fcab187. PubMed
  5. Jagodinsky JC, et al. 2022. J Immunother Cancer. 10:. PubMed
  6. Zhou LJ et al. 2019. Cell Rep. 27(13):3844-3859 . PubMed
  7. Liu TC, et al. 2021. Cell Host Microbe. 29:988. PubMed
  8. Meng W, et al. 2021. JCI Insight. 6:. PubMed
  9. Hayes BH, et al. 2020. J Cell Sci. . PubMed
  10. Bodduluri SR, et al. 2018. Cancer Immunol Res. 6(3). PubMed
  11. Miller JE, et al. 2020. Front Immunol. 11:108. PubMed
  12. Moon JS, et al. 2021. JCI Insight. 6:. PubMed
  13. Wang Q, et al. 2022. Nat Commun. 13:3022. PubMed
  14. Adachi Y, et al. 2022. Nat Commun. 13:5117. PubMed
  15. Torretta S, et al. 2020. Nat Commun. 4.863194444. PubMed
  16. Li Y, et al. 2022. J Lipid Res. :100273. PubMed
  17. Feng J, et al. 2017. Cell Mol Immunol. 10.1038/cmi.2016.71. PubMed
  18. Zhou Y, et al. 2020. Hepatology. 71:1453. PubMed
  19. Cox N, et al. 2015. Proc Natl Acad Sci U S A. 112: 8385 - 8390. PubMed
  20. Hammer A, et al. 2016. Proc Natl Acad Sci U S A. 113(49):14109-14114. PubMed
  21. Hu J, et al. 2020. Theranostics. 7.154166667. PubMed
  22. Woo YD, et al. 2020. J Allergy Clin Immunol. . PubMed
  23. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  24. Ikeda S, et al. 2022. Sci Rep. 12:11564. PubMed
  25. Dabouz R, et al. 2020. J Neuroinflammation. 0.957638889. PubMed
  26. Miranda K, et al. 2019. Front Immunol. 10:1049. PubMed
  27. Tian J, et al. 2021. EMBO J. 40:e106065. PubMed
  28. Wang X, et al. 2021. Cell Death Differ. 28:3235. PubMed
  29. Lee D, et al. 2021. J Lipid Res. 62:100117. PubMed
  30. Dongre A, et al. 2021. Cancer Discov. 11:1286. PubMed
  31. Vorselen D, et al. 2021. Elife. 10:. PubMed
  32. Mouton AJ, et al. 2021. J Mol Cell Cardiol. 158:38. PubMed
  33. Thi Tran U, et al. 2019. Commun Biol. 2:2. PubMed
  34. Saika R, et al. 2017. Journal of Neuroinflammation . 10.1186/s12974-017-0884-8. PubMed
  35. Chen Y, et al. 2021. Front Pharmacol. 12:735194. PubMed
  36. Hao Q, et al. 2022. Cells. 11:. PubMed
  37. Zhang T, et al. 2021. Redox Biol. 101930:41. PubMed
  38. Huber R, et al. 2015. PLoS One. 10: 0144338. PubMed
  39. Lu Y, et al. 2020. Immunity. 52:782. PubMed
  40. Hayes BH, et al. 2020. J Cell Sci. 133:00:00. PubMed
  41. Li X, et al. 2019. Cell Death Discov. 5:62. PubMed
  42. Miyamoto T, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  43. Yang Z, et al. 2017. J Biol Chem. 292(4):1178-1186. PubMed
  44. Miranda K, et al. 2018. Int J Obes (Lond). 42:1140. PubMed
  45. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  46. Olmsted-Davis E, et al. 2021. Front Immunol. 12:686769. PubMed
  47. Huffaker TB, et al. 2021. Nat Commun. 12:2620. PubMed
  48. Schröder A, et al. 2021. Int J Mol Sci. 22:00. PubMed
  49. Nakayama Y, et al. 2020. Proc Natl Acad Sci U S A. 117:14365. PubMed
  50. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  51. Woods PS, et al. 2020. Am J Respir Cell Mol Biol. 62:243. PubMed
  52. Wang Y, et al. 2019. Front Cell Infect Microbiol. 9:286. PubMed
  53. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  54. Gomez-Lopez N, et al. 2021. JCI Insight. 6:. PubMed
  55. Davies JM, et al. 2021. Immunol Cell Biol. 99:622. PubMed
  56. Zhao H, et al. 2022. Front Immunol. 13:931087. PubMed
  57. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
  58. Chen YH, et al. 2022. Arthritis Res Ther. 24:27. PubMed
  59. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed

Antigen Details

Disulfide-linked glycosylated homodimer.

M-CSF is broadly expressed in adult mouse tissues. M-CSF is released by fibroblasts, breast cancer cell lines, alveolar macrophages, stromal bone marrow cells, endothelial cells, and mesenchymal cells.

M-CSF is the key regulator of the survival, proliferation, and differentiation of mononuclear phagocytes and plays a central role in the regulation of osteoclastogenesis. CSF-1 also regulates the development of Paneth cells, Langerhans cells, lamina propria dendritic cells, and microglia.
Monocytes, macrophages, mononuclear phagocyte precursors, microglia, proliferating smooth muscle cells, umbilical vein endothelial cells, and breast cancer cell lines.
M-CSFR or CSF1R (CD115)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Cell Proliferation and Viability, Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Kawasaki ES, et al. 1985. Science 230:291.
2. Wei S, et al. 2010. J. Leukocyte Biol. 88:495.
3. MacDonald KP, et al. 2010. Blood 116:3955.
4. Hodge JM, et al. 2011. Plos One 6:e21462.
5. Morandi et al. 2011. Plos One 6:e27450.
6. Erblich B, et al. 2011. Plos One 6:e26317.

Gene ID
12977 View all products for this Gene ID
View information about M-CSF on

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 3    Revision Date: 01-17-2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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