Recombinant Mouse GM-CSF (carrier-free)

Pricing & Availability
Other Names
Granulocyte/macrophage-colony stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)
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Product Citations
publications
GM-CSF_Mouse_Recombinant_Protein_BA_031313
FDC-P1 cell proliferation induced by mouse GM-CSF.
  • GM-CSF_Mouse_Recombinant_Protein_BA_031313
    FDC-P1 cell proliferation induced by mouse GM-CSF.
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Cat # Size Price Quantity Avail. Save
576302 10 µg 100€
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576304 25 µg 172€
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576306 100 µg 520€
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576308 500 µg 1.360€
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Description

GM-CSF is a hematopoietic factor that is produced by T cells, macrophages, fibroblasts and endothelial cells. This multifunctional cytokine stimulates progenitor cells of neutrophils, eosinophils and macrophages. GM-CSF is also a differentiation and activating factor for granulocytic and monocytic cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse GM-CSF, amino acids Ala18-Lys141 (Accession # NM_009969) was expressed in E. coli.
Molecular Mass
The 125 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 14.2 kDa. The DTT-reduced protein migrates at approximately 13 kDa and the non-reduced protein migrates at approximately 11kDa by SDS-PAGE.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is <0.05 ng/ml, corresponding to a specific activity >2x107 units/mg.
Application

Bioassay

Recommended Usage

Use when high specific biological activity is required.

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Application References
  1. Ahn J, et al. 2012. PNAS. 109:19386. PubMed
  2. Verhagen J, et al. 2012. PNAS. 110:E221. PubMed
  3. Bretscher P, et al. 2015. EMBO Mol Med. 7:593. PubMed
Product Citations
  1. Ayala M, et al. 2017. J Cancer Res Clin Oncol. 10.1007/s00432-017-2421-7. PubMed
  2. Ayala M, et al. 2017. Breast Cancer Res Treat. 10.1007/s10549-017-4414-2. PubMed
  3. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  4. Nakamura K, et al. 2018. Cancer Cell. 33:634. PubMed
  5. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  6. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  7. Wang Y, et al. 2019. Front Cell Infect Microbiol. 9:286. PubMed
  8. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  9. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  10. Chowdhury D, et al. 2019. Cell Rep. 27:3873. PubMed
  11. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  12. Gogokhia L, et al. 2019. Cell Host Microbe. 25:285. PubMed
  13. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  14. Lajqi T, et al. 2020. Front Immunol. 11:546415. PubMed
  15. Ahn J, et al. 2012. Proc Natl Acad Sci U S A. 109:19386. PubMed
  16. Bretscher P, et al. 2015. EMBO Mol Med. 7:593. PubMed
  17. Jin Z, et al. 2020. NPJ Regen Med. 5:19. PubMed
  18. Markworth JF, et al. 2020. JCI Insight. 5:00. PubMed
  19. Alban TJ, et al. 2020. Front Immunol. 1.285416667. PubMed
  20. Delbridge ARD, et al. 2021. Front Cell Neurosci. 14:592005. PubMed

Antigen Details

Structure
cytokine
Distribution

T cells, monocytes/macrophages, fibroblasts, endothelial cells, mast cells

Function
Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans
Interaction
Granulocyte/macrophage/erythroid/megakaryocytic progenitors, myeloblasts, monoblasts
Ligand/Receptor
Heterodimer GM-CSFR µ subunit (CDw116); β-subunit (CDw131) in common with IL-3R, IL-5R
Bioactivity
Growth/development granulocyte/macrophage progenitors; differentiates myeloblasts/monoblasts; synergizes with Epo proliferation of erythroid/megakaryocytic progenitors
Cell Type
Hematopoietic stem and progenitors, Embryonic Stem Cells
Biology Area
Cell Biology, Stem Cells
Molecular Family
Growth Factors, Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Demetri G, et al. 1991. Blood 78:2791.
3. Fan D, et al. 1991. In vivo 5:571.
4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.

Gene ID
12981 View all products for this Gene ID
UniProt
View information about GM-CSF on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
Go To Top Version: 5    Revision Date: 08-19-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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