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Purified anti-mouse CD28 Antibody

Pricing & Availability
Clone
37.51 (See other available formats)
Regulatory Status
RUO
Other Names
Tp44, T44
Isotype
Syrian Hamster IgG
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Product Citations
publications
37.51
C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
  • 37.51
    C57BL/6 splenocytes stained with 37.51 PE and CD3 FITC
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102101 50 µg 44€
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102102 500 µg 102€
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Description

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD28 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, IHC-F, Costim, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).

Application References
  1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
  2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
  3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
  4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
  5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
  6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
  8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
  9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
  10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  11. Barbi J, et al. 2007. Blood 110:2215.
  12. Milpied P, et al. 2011. Blood 118:2993. PubMed
  13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
  14. Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
  15. Li CR, et al. 2014. J Immunol. 192:1425. PubMed
  16. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. Sandu I, et al. 2020. Nat Commun. 11:4454. PubMed
  2. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  3. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  4. Kim K, et al. 2016. PLoS One. 11: 0148576. PubMed
  5. Akiyama C, et al. 2019. J Immunol. 203:835. PubMed
  6. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  7. Oh H, et al. 2017. Immunity. 47:450. PubMed
  8. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  9. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  10. Cross EW, et al. 2019. PLoS One. 14:e0211446. PubMed
  11. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  12. Hawse WF, et al. 2019. J Immunol. 203:2771. PubMed
  13. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  14. Busnelli M, et al. 2022. Arterioscler Thromb Vasc Biol. 42:839. PubMed
  15. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  16. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  17. Yamada T, et al. 2019. J Immunol. 202:1088. PubMed
  18. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  19. He N 2005. J Immunol. 174:6967. PubMed
  20. Crispin J, et al. 2012. J Immunol. 188:3567. PubMed
  21. Milpied P, et al. 2011. Blood. 118:2993. PubMed
  22. Sanctuary MR, et al. 2019. Mucosal Immunol. 0.638888889. PubMed
  23. Rasoulouniriana D, et al. 2019. J Clin Invest. 129:4151. PubMed
  24. Oghumu S, et al. 2019. J Immunol. 203:789. PubMed
  25. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  26. Macchi C, et al. 2022. iScience. 25:104435. PubMed
  27. Lu SX, et al. 2021. Cell. . PubMed
  28. Kitamura T, et al. 2018. Front Immunol. 8:2004. PubMed
  29. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
  30. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  31. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  32. Marangoni F, et al. 2021. Cell. . PubMed
  33. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
  34. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  35. Kölle J, et al. 2022. iScience. 25:104440. PubMed
  36. Montes de Oca M, et al. 2020. PLoS Pathog. 16:e1008994. PubMed
  37. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  38. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  39. Tuzlak S, et al. 2017. Cell Death Differ. 24:523. PubMed
  40. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  41. Rao TN, et al. 2020. J Immunol. 204:2600. PubMed
  42. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  43. Perchonock C, et al. 2006. Mol Cell Biol . 26:6005. PubMed
  44. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  45. Srey MT, et al. 2020. PLoS Negl Trop Dis. 14:e0008842. PubMed
  46. Hanoteau A, et al. 2019. J Immunother Cancer. 7:10. PubMed
  47. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  48. Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
  49. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  50. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  51. Morales-Mantilla DE, et al. 2022. Elife. 11:. PubMed
  52. McCully M, et al. 2015. J Immunol. 195: 96 - 104. PubMed
  53. Takushi SE, et al. 2020. Hum Gene Ther. 31:626. PubMed
  54. Rosenberger C, et al. 2008. Proc Natl Acad Sci U S A. 105:2544. PubMed
  55. Racioppi L, et al. 2019. Nat Commun. 10:2450. PubMed
  56. Miret JJ, et al. 2019. J Immunother Cancer. 7:32. PubMed
  57. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  58. Otsuka S, et al. 2021. J Clin Invest. 131:. PubMed
  59. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  60. Scholz T, et al. 2017. PLoS One. 12:e0182646. PubMed
  61. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  62. Li Z, et al. 2021. MBio. 12:e0254221. PubMed
  63. Zhang S, et al. 2021. Front Immunol. 12:635076. PubMed
  64. Samanta D, et al. 2020. Cell Rep. 108073:32. PubMed
  65. Hollmann C, et al. 2016. J Immunol. 197: 3130 - 3141. PubMed
  66. Thumkeo D, et al. 2020. Sci Adv. 6:eaay2432. PubMed
  67. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  68. Hao Y, et al. 2018. Front Immunol. 9:1127. PubMed
  69. Sripada A, et al. 2021. PLoS Biol. 19:e3001063. PubMed
  70. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  71. Wu Y, et al. 2021. J Clin Invest. 131:. PubMed
  72. Schreiber K, et al. 2020. Cancer Immunol Res. 8:192. PubMed
  73. Endo Y, et al. 2015. Cell Rep. 12: 1042-1055. PubMed
  74. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  75. Majchrzak K, et al. 2017. JCI Insight. 2:e90547. PubMed
  76. Patterson AR, et al. 2019. J Allergy Clin Immunol. 143:245. PubMed
  77. Klein JC, et al. 2017. Nat Commun. 8:14600. PubMed
  78. Lee J, et al. 2020. Cells. 9:. PubMed
  79. Pan S, et al. 2021. Front Cell Dev Biol. 9:629306. PubMed
  80. Schiering C, et al. 2017. Nature. 542:242. PubMed
  81. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  82. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  83. Link MA, et al. 2017. PLoS One. 12:e0184320. PubMed
  84. Sun Y, et al. 2021. Nat Commun. 12:5147. PubMed
  85. Guo J, et al. 2020. Cell Mol Immunol. 1222:17. PubMed
  86. Rosenberg J, et al. 2020. Cell Systems. 10(5):433-444. PubMed
  87. Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed
  88. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  89. Nakazawa Y, et al. 2021. Elife. 10:. PubMed
  90. Cohen MA, et al. 2020. Cell Stem Cell. 26(4):579-592. PubMed
  91. He N 2005. J Exp Med. 202:395. PubMed
  92. Cunningham N, et al. 2011. Int Immunol. 1.439583333. PubMed
  93. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  94. He Y, et al. 2021. eLife. 10:00. PubMed
  95. Perchonock C, et al. 2007. J Immunol. 179:1768. PubMed
  96. Abe Y, et al. 2017. Biochem Biophys Rep. 0.543055556. PubMed
  97. Robles-Valero J, et al. 2021. EMBO J. 40:e108125. PubMed
  98. Wang W, et al. 2022. Nat Commun. 13:4495. PubMed
  99. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  100. Bessell CA, et al. 2020. JCI Insight. 5:. PubMed
  101. Chen YH, et al. 2022. Arthritis Res Ther. 24:27. PubMed
  102. Xia L, et al. 2021. Cancer Immunol Res. 9:707. PubMed
  103. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  104. Plaza-Sirvent C, et al. 2017. Cell Rep. 18(1):12-22. PubMed
  105. Grinberg–Bleyer Y, et al. 2017. Cell. 170:1096. PubMed
RRID
AB_312866 (BioLegend Cat. No. 102101)
AB_312867 (BioLegend Cat. No. 102102)
Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution

Thymocytes, CD4+, CD8+ peripheral T cells, NK cells

Function
Costimulates T and NK cells
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.

Gene ID
12487 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org
Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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