Purified anti-LIF Antibody

Pricing & Availability
Clone
M1506B09 (See other available formats)
Other Names
Leukemia inhibitory factor, HILDA (human interleukin in DA cells), MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor)
Isotype
Rat IgG2a
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B_M1506B09_Purified_LIF_Antibody_WB_2_012615
Western blot analysis of untreated PBMC (lane 1), PBMC activated with 5 µg/mL of plate-coated anti-CD3 antibody and 2 µg/mL of anti-CD28 antibody for two days (lane 2), untreated IMR-32 cells (lane 3), untreated Raw264.7 (lane 4), and Raw264.7 starved overnight and treated with 1 µg/mL of LPS overnight (lane 5). Anti-LIF antibody (clone M1506B09) and anti-β actin antibody (poly6221) were used, followed by HRP anti-rat IgG antibody (or HRP anti-rabbit IgG (minimal x-reactivity) antibody.
  • B_M1506B09_Purified_LIF_Antibody_WB_2_012615
    Western blot analysis of untreated PBMC (lane 1), PBMC activated with 5 µg/mL of plate-coated anti-CD3 antibody and 2 µg/mL of anti-CD28 antibody for two days (lane 2), untreated IMR-32 cells (lane 3), untreated Raw264.7 (lane 4), and Raw264.7 starved overnight and treated with 1 µg/mL of LPS overnight (lane 5). Anti-LIF antibody (clone M1506B09) and anti-β actin antibody (poly6221) were used, followed by HRP anti-rat IgG antibody (or HRP anti-rabbit IgG (minimal x-reactivity) antibody.
  • C_M1506B09_PURE_LIF_IF_032318
    HeLa cells were fixed with ice cold methanol for six minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/mL anti‐LIF (clone M1506B09) antibody followed by Alexa Fluor® 594 conjugated anti‐rat IgG (red) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 40X objective.
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674702 100 µg 156€
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Description

Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190, and the subunit gp130. The LIF complex receptor signals though STAT3 and JAK kinases. LIF expression has been observed in various tissues including the thymus, lung, and neuronal tissue. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contributes to stem cell self-renewal. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, as well as the induction of neuronal cell differentiation. It also regulates mesenchymal to epithelial conversion during kidney development, and also may have a role in immune tolerance at the maternal-fetal interface. LIF can be upregulated by pro-inflammatory cytokines such as TNF-α and IL-17. Elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse LIF produced in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 2.0 µg per mL. For immunocytochemistry, a concentration range of 2.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565278 (BioLegend Cat. No. 674702)

Antigen Details

Structure
202 amino acids with a predicted molecular weight of approximately 22 kD.
Distribution

Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.

Function
Inhibits embryonic stem cell differentation and induces the terminal differentiation of myeloid leukemia cells. LIF is upregulated by TNF-α and IL-17. LIF is downregulated by IL-4 and IL-13 in fibroblasts.
Interaction
Embryonic stem cells.
Ligand/Receptor
LIF receptor complex (gp190 and gp130).
Cell Type
T cells, Tregs
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Moreau JF, et al. 1988. Nature 336:690.
2. Tomida M, et al. FEBS Lett. 334:193.
3. Metcalfe SM. 2011. Genes Immun. 12:157.
4. Voyle RB, et al. 1999. Exp. Cell Res. 249:199.
5. Slaets H, et al. 2010. Trends Mol. Med. 16:493.
6. Souza PP, et al. 2012. Mol. Immunol. 49:601

Gene ID
3976 View all products for this Gene ID
UniProt
View information about LIF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06-01-2016

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