Alexa Fluor® 594 anti-LIF Antibody

Pricing & Availability
Clone
M1506B09 (See other available formats)
Other Names
Leukemia inhibitory factor, HILDA (human interleukin in DA cells), MLPLI (melanoma-derived LPL inhibitor), DIA (differentiation-inducing factor)
Isotype
Rat IgG2a
Ave. Rating
Submit a Review
Product Citations
publications
M1506B09_AF594_LIF_Antibody_IF_073115
HeLa cells were fixed with ice cold methanol for five minutes and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/ml Alexa Fluor® 594 conjugated (red) LIF Antibody (clone M1506B09) in a blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • M1506B09_AF594_LIF_Antibody_IF_073115
    HeLa cells were fixed with ice cold methanol for five minutes and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 2 µg/ml Alexa Fluor® 594 conjugated (red) LIF Antibody (clone M1506B09) in a blocking buffer overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
674704 100 µg 228€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the IL-6 receptor family. It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190, and the subunit gp130. The LIF complex receptor signals though STAT3 and JAK kinases. LIF expression has been observed in various tissues including the thymus, lung, and neuronal tissue. LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contributes to stem cell self-renewal. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, as well as the induction of neuronal cell differentiation. It also regulates mesenchymal to epithelial conversion during kidney development, and also may have a role in immune tolerance at the maternal-fetal interface. LIF can be upregulated by pro-inflammatory cytokines such as TNF-α and IL-17. Elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse LIF produced in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
RRID
AB_2566178 (BioLegend Cat. No. 674704)

Antigen Details

Structure
202 amino acids with a predicted molecular weight of approximately 22 kD.
Distribution

Expressed in the trophectoderm of the developing embryo, activated CD4+ T cells.

Function
Inhibits embryonic stem cell differentation and induces the terminal differentiation of myeloid leukemia cells. LIF is upregulated by TNF-α and IL-17. LIF is downregulated by IL-4 and IL-13 in fibroblasts.
Interaction
Embryonic stem cells.
Ligand/Receptor
LIF receptor complex (gp190 and gp130).
Cell Type
T cells, Tregs
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines, Growth Factors
Antigen References

1. Moreau JF, et al. 1988. Nature 336:690.
2. Tomida M, et al. FEBS Lett. 334:193.
3. Metcalfe SM. 2011. Genes Immun. 12:157.
4. Voyle RB, et al. 1999. Exp. Cell Res. 249:199.
5. Slaets H, et al. 2010. Trends Mol. Med. 16:493.
6. Souza PP, et al. 2012. Mol. Immunol. 49:601

Gene ID
3976 View all products for this Gene ID
UniProt
View information about LIF on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07-31-2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account