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Purified anti-human CD45RO Antibody

Pricing & Availability
Clone
UCHL1 (See other available formats)
Regulatory Status
RUO
Workshop
IV N31
Other Names
CD45RO
Isotype
Mouse IgG2a, κ
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Product Citations
publications
UCHL1_Purified_062707
Human peripheral blood lymphocytes stained with purified UCHL1, followed by anti-mouse IgGs FITC
  • UCHL1_Purified_062707
    Human peripheral blood lymphocytes stained with purified UCHL1, followed by anti-mouse IgGs FITC
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304202 100 µg 40€
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Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee, Cynomolgus, Common Marmoset
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
IL-2 dependent T cell line, CA1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, IP, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.

Application References
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
  2. Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
  3. Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
  4. Yamada M, et al. 1996. Stroke 27:1155. (IHC)
  5. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
  6. Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
  7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  8. Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
  9. Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
  10. Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
  11. Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
  2. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  3. Delgobo M, et al. 2021. Front Immunol. 12:584538. PubMed
  4. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  5. Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
  6. Ferrian S, et al. 2021. Cell Rep Med. 2:100419. PubMed
  7. Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
  8. Guo T, et al. 2018. J Immunol. 200:500. PubMed
  9. Cosgrove PR, et al. 2021. Pediatr Res. . PubMed
  10. Wu Y, et al. 2007. Infect Immun. 75:4357. PubMed
  11. Xu-Monette ZY, et al. 2019. Cancer Immunol Res. 7:644. PubMed
  12. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  13. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  14. Gee MH, et al. 2018. Cell. 172:549. PubMed
  15. Mozaffarian N, et al. 2008. Rheumatology. 47:1335. PubMed
  16. Wu Y, et al. 2007. Infect Immun . 178:2802. PubMed
  17. Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
  18. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  19. Sergio Gonzalez‐Duque et al. 2018. Cell metabolism. 28(6):946-960 . PubMed
  20. NULL, et al. 2022. Cell. 185:916. PubMed
  21. White M, et al. 2015. J Immunol. 195: 1858-1867. PubMed
  22. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  23. Bender C, et al. 2020. Sci Adv. :6. PubMed
  24. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  25. Martin E, et al. 2020. JCI Insight. :5. PubMed
  26. Li Z, et al. 2020. J Clin Lab Anal. 34:e23155. PubMed
RRID
AB_314418 (BioLegend Cat. No. 304202)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution

Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
CD22
Cell Type
B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RO on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45RO Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD45RO UCHL1 FC
FITC anti-human CD45RO UCHL1 FC
PE anti-human CD45RO UCHL1 FC
PE/Cyanine5 anti-human CD45RO UCHL1 FC
Purified anti-human CD45RO UCHL1 FC,WB,IP,IHC-P
Alexa Fluor® 488 anti-human CD45RO UCHL1 FC,IHC-P
Pacific Blue™ anti-human CD45RO UCHL1 FC
Alexa Fluor® 700 anti-human CD45RO UCHL1 FC
Biotin anti-human CD45RO UCHL1 FC
Brilliant Violet 421™ anti-human CD45RO UCHL1 FC,IHC-P
PerCP/Cyanine5.5 anti-human CD45RO UCHL1 FC
Brilliant Violet 570™ anti-human CD45RO UCHL1 FC
Brilliant Violet 605™ anti-human CD45RO UCHL1 FC
APC/Cyanine7 anti-human CD45RO UCHL1 FC
PE/Cyanine7 anti-human CD45RO UCHL1 FC
Brilliant Violet 650™ anti-human CD45RO UCHL1 FC
Brilliant Violet 711™ anti-human CD45RO UCHL1 FC
Brilliant Violet 785™ anti-human CD45RO UCHL1 FC
Alexa Fluor® 594 anti-human CD45RO UCHL1 IHC-P
Purified anti-human CD45RO (Maxpar® Ready) UCHL1 FC,CyTOF®
Brilliant Violet 510™ anti-human CD45RO UCHL1 FC
PE/Dazzle™ 594 anti-human CD45RO UCHL1 FC
APC/Fire™ 750 anti-human CD45RO UCHL1 FC
PerCP anti-human CD45RO UCHL1 FC
APC anti-human CD45RO UCHL1 FC
TotalSeq™-A0087 anti-human CD45RO UCHL1 PG
TotalSeq™-B0087 anti-human CD45RO UCHL1 PG
TotalSeq™-C0087 anti-human CD45RO UCHL1 PG
Brilliant Violet 750™ anti-human CD45RO UCHL1 FC
PE/Fire™ 640 anti-human CD45RO UCHL1 FC
TotalSeq™-D0087 anti-human CD45RO UCHL1 PG
Spark Violet™ 423 anti-human CD45RO UCHL1 FC
GMP APC anti-human CD45RO UCHL1 FC
PE anti-human CD45RO UCHL1 FC
Go To Top Version: 3    Revision Date: 08-31-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-human CD45RO

  • FITC anti-human CD45RO

  • PE anti-human CD45RO

  • PE/Cyanine5 anti-human CD45RO

  • Purified anti-human CD45RO

  • Alexa Fluor® 488 anti-human CD45RO

  • Pacific Blue™ anti-human CD45RO

  • Alexa Fluor® 700 anti-human CD45RO

  • Biotin anti-human CD45RO

  • Brilliant Violet 421™ anti-human CD45RO

  • PerCP/Cyanine5.5 anti-human CD45RO

  • Brilliant Violet 570™ anti-human CD45RO

  • Brilliant Violet 605™ anti-human CD45RO

  • APC/Cyanine7 anti-human CD45RO

  • PE/Cyanine7 anti-human CD45RO

  • Brilliant Violet 650™ anti-human CD45RO

  • Brilliant Violet 711™ anti-human CD45RO

  • Brilliant Violet 785™ anti-human CD45RO

  • Alexa Fluor® 594 anti-human CD45RO

  • Purified anti-human CD45RO (Maxpar® Ready)

  • Brilliant Violet 510™ anti-human CD45RO

  • PE/Dazzle™ 594 anti-human CD45RO

  • APC/Fire™ 750 anti-human CD45RO

  • PerCP anti-human CD45RO

  • APC anti-human CD45RO

  • TotalSeq™-A0087 anti-human CD45RO

  • TotalSeq™-B0087 anti-human CD45RO

  • TotalSeq™-C0087 anti-human CD45RO

  • Brilliant Violet 750™ anti-human CD45RO

  • PE/Fire™ 640 anti-human CD45RO

  • TotalSeq™-D0087 anti-human CD45RO

  • Spark Violet™ 423 anti-human CD45RO

  • GMP APC anti-human CD45RO

  • PE anti-human CD45RO
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