Loading...

Purified anti-human CD34 Antibody

Pricing & Availability
Clone
581 (See other available formats)
Regulatory Status
RUO
Workshop
V MA27
Other Names
Gp105-120, My10
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
581_Pure_030510
Human myeloid cell line KG1a stained with purified 581, followed by anti-mouse IgG FITC
  • 581_Pure_030510
    Human myeloid cell line KG1a stained with purified 581, followed by anti-mouse IgG FITC
Compare all formats
Cat # Size Price Quantity Check Availability Save
343501 25 µg 48€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
343502 100 µg 92€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
ICC, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.

Application References
  1. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press.
  2. Felschow DM, et al. 2001. Blood 97:3768.
  3. Rudin CE, et al. 1997. Br. J. Haematol. 97:488.
  4. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  5. Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC)
  6. Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations
  1. Liu C, et al. 2022. Regen Ther. 21:192. PubMed
  2. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  3. Zhou W et al. 2017. Cell stem cell. 21(5):591-603 . PubMed
  4. Henrick BM, et al. 2021. Cell. . PubMed
  5. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  6. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  7. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  8. Richmond BW, et al. 2020. Mucosal Immunol. . PubMed
  9. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  10. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  11. Crawford LB, et al. 2021. J Virol. 95:. PubMed
RRID
AB_1731969 (BioLegend Cat. No. 343501)
AB_1731898 (BioLegend Cat. No. 343502)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 08-23-2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD34

  • FITC anti-human CD34

  • PE anti-human CD34

  • Alexa Fluor® 647 anti-human CD34

  • APC anti-human CD34

  • Pacific Blue™ anti-human CD34

  • APC/Cyanine7 anti-human CD34

  • PE/Cyanine7 anti-human CD34

  • Alexa Fluor® 488 anti-human CD34

  • PerCP anti-human CD34

  • PerCP/Cyanine5.5 anti-human CD34

  • Biotin anti-human CD34

  • Alexa Fluor® 700 anti-human CD34

  • Brilliant Violet 510™ anti-human CD34

  • Purified anti-human CD34 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-human CD34

  • APC/Fire™ 750 anti-human CD34

  • TotalSeq™-A0054 anti-human CD34

  • TotalSeq™-B0054 anti-human CD34

  • TotalSeq™-C0054 anti-human CD34

  • TotalSeq™-D0054 anti-human CD34

  • Spark Red™ 718 anti-human CD34
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account