Purified anti-human CD28 Antibody

Pricing & Availability
Clone
CD28.2 (See other available formats)
Regulatory Status
RUO
Workshop
V-CD28.05
Other Names
T44, Tp44
Isotype
Mouse IgG1, κ
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Product Citations
publications
28.2
Human peripheral blood lymphocytes stained with CD28.2 PE
  • 28.2
    Human peripheral blood lymphocytes stained with CD28.2 PE
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302901 25 µg 16,00€
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302902 100 µg 36,00€
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Description

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, Costim, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for highly sensitive assays.

Application References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Nunes J, et al. 1993. Biochem. J. 293:835.
  3. Calea-Lauri J, et al. 1999. J. Immunol. 163:62.
  4. Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC)
  5. Marti F, et al. 2001. J. Immunol. 166:197. (Costim)
  6. Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
  7. Rivollier A, et al. 2004. Blood 104:4029. (Costim)
  8. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim)
  9. Sheng W, et al. 2007.Elsevier 580:6819. PubMed
  10. Mitsuhashi M. 2007. Clin Chem.53:148. PubMed
  11. Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed
  12. Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed
  13. Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC)
  14. Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed
  15. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  16. Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed
  17. Nomura T, et al. 2012. J. Virol. 86:6481. PubMed
Product Citations
  1. Jones N, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01516. PubMed
  2. Qiu H, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.08.036. PubMed
  3. Jin Y, et al. 2017. Int Immunopharmacol. . 10.1016/j.intimp.2017.10.009. PubMed
  4. Pawlak EN, et al. 2018. Retrovirology. 15:6. PubMed
  5. Bengsch B et al. 2018. Immunity. 48(5):1029-1045 . PubMed
  6. Pardons M, et al. 2019. PLoS Pathog. 15:e1007619. PubMed
  7. Zhu Y, et al. 2019. Front Genet. 10:141. PubMed
  8. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  9. Zhang Y, et al. 2019. Cell Res. 29:609. PubMed
  10. Mazer M, et al. 2019. J Immunol. 203:2088. PubMed
  11. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  12. Bantug GR, et al. 2018. Immunity. 48:542. PubMed
  13. Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
  14. Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed
  15. Guo T, et al. 2018. J Immunol. 200:500. PubMed
  16. Gee MH, et al. 2018. Cell. 172:549. PubMed
  17. Dias J, et al. 2018. Proc Natl Acad Sci U S A. 115:E11513. PubMed
  18. Jin H, et al. 2019. MBio. 10:e01769-19. PubMed
  19. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  20. Seo N, et al. 2018. Nat Commun. 9:435. PubMed
  21. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  22. Zucchetti AE, et al. 2019. Nat Commun. 10:2864. PubMed
  23. Bluhm J, et al. 2018. Mol Ther. 26:1906. PubMed
  24. Bobardt M, et al. 2020. PLoS One. 15:e0227715. PubMed
  25. Holbrook AK, et al. 2019. Physiol Rep. 7:e14234. PubMed
  26. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  27. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
  28. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  29. Mousset CM, et al. 2018. Oncoimmunology. 7:e1488565. PubMed
  30. Oberg HH, et al. 2020. J Leukoc Biol. 107:1081. PubMed
  31. Sheng WY, et al. 2006. FEBS Lett. 580:6819. PubMed
  32. Mitsuhashi M 2007. Clin Chem. 53:148. PubMed
  33. Ye Z, et al. 2008. Infect Immun. 76:2541. PubMed
  34. Leonard J, et al. 2011. J Virol. 85:6867. PubMed
  35. Nomura T, et al. 2012. J Virol. 86:6481. PubMed
  36. Haas R, et al. 2015. PLoS Biol. 13: 1002202. PubMed
  37. Liu L, et al. 2016. Nat Biotechnol. 10.1038/nbt.3461. PubMed
  38. Barzilai S, et al. 2017. Cell Rep. 18(3):685-699. PubMed
  39. Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
  40. Zavidij O, et al. 2020. Nat Cancer. 0.384027778. PubMed
  41. Hu X, et al. 2021. British Journal of Pharmacology. 178(6):1445-1458. PubMed
  42. Cai J, et al. 2021. eLife. 10:00. PubMed
  43. He Y, et al. 2021. eLife. 10:00. PubMed
  44. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  45. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  46. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  47. Nathan A, et al. 2021. Cell. 184(17):4401-4413.e10. PubMed
  48. Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
  49. Martin E, et al. 2020. JCI Insight. :5. PubMed
  50. Panigrahi S, et al. 2020. PLoS Pathog. e1008885:16. PubMed
  51. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
RRID
AB_314303 (BioLegend Cat. No. 302901)
AB_314304 (BioLegend Cat. No. 302902)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution

Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells

Function
T cell costimulation
Ligand/Receptor
CD80, CD86
Cell Type
B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. June CH, et al. 1994. Immunol. Today 15:321.
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.

Gene ID
940 View all products for this Gene ID
UniProt
View information about CD28 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07-13-2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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