Purified anti-human CD134 (OX40) (Maxpar® Ready) Antibody

Pricing & Availability
Clone
Ber-ACT35 (ACT35) (See other available formats)
Regulatory Status
RUO
Other Names
ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
Isotype
Mouse IgG1, κ
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Product Citations
publications
ACT35_Purified_CD134_Cytof_1_060314.jpg
Human PBMCs were incubated for 3 days in media alone (top) or with PHA (bottom) and then stained with 154Sm-anti-CD45 (HI30) and 158Gd-anti-CD134 (ACT35). CD45+CD14- cells are displayed in the analysis. Data provided by DVS Sciences.
  • ACT35_Purified_CD134_Cytof_1_060314.jpg
    Human PBMCs were incubated for 3 days in media alone (top) or with PHA (bottom) and then stained with 154Sm-anti-CD45 (HI30) and 158Gd-anti-CD134 (ACT35). CD45+CD14- cells are displayed in the analysis. Data provided by DVS Sciences.
  • ACT35_Purified_CD134_Cytof_2_060314.jpg
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350015 100 µg 100,00€
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Description

CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4+ T cell tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HTLV 1-transformed HUT 102 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated to simplify the antibody preparation when performing the labeling protocol. As a result, it is possible to proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, immunohistochemical staining2,3of paraffin embedded7 and frozen tissue sections, ELISA4, and functional assay5. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).

Additional Product Notes
Maxpar® is a registered trademark of Fluidigm Inc.
Application References
  1. Latza U, et al. 1994. Eur. J. Immunol. 24:677. (WB, IP)
  2. Durkop H, et al. 1995. Brit. J. Haematol. 91:927. (IHC)
  3. Durkop H, et al. 1997. Brit. J. Haematol. 98:863. (IHC)
  4. Willett B, et al. 2007. J. Virol. 81:9665. (ELISA)
  5. Li M and Zhang Y. et al. 2005. Cell. Mol. Immunol. 2:467. (FA)
  6. Gloviczki ML, et al. 2012. Clin. J. Am. Soc. Nephrol. 8:546. PubMed
  7. Domingos PL, et al. 2012. An. Bras. Dermatol. 87:851. (IHC)
Product Citations
  1. Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
RRID
AB_2563718 (BioLegend Cat. No. 350015)

Antigen Details

Structure
50 kD, TNF receptor family
Distribution

Activated T cells

Function
Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
Ligand/Receptor
OX40L (CD252)
Cell Type
T cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Smith CA, et al. 1994. Cell. 76:959.
2. Chen AL, et al. 1999. Immunity. 11:689.
3. Croft M. 2010. Annu. Rev. Immunol. 28:57.
4. Ruby CE, et al. 2009. J. Immunol. 183:5079.
5. Klinger M, et al. 2009. J. Immunol. 182:4581.

Gene ID
7293 View all products for this Gene ID
UniProt
View information about CD134 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Go To Top Version: 0    Revision Date: 07-30-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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