PE anti-mouse TCR Vα2 Antibody

Pricing & Availability
Clone
B20.1 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vα 2
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
B20dot1_PE_030209.jpg
C57BL/6 splenocytes stained with 145-2C11 (CD3) APC and B20.1 PE
  • B20dot1_PE_030209.jpg
    C57BL/6 splenocytes stained with 145-2C11 (CD3) APC and B20.1 PE
See PE spectral data
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127807 25 µg 64,00€
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127808 100 µg 140,00€
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Description

The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble TCR from mouse CTL clone KB5-C20
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.

Application References
  1. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
  2. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
  3. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
  4. Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed
  5. Rao RR, et al. 2012. Immunity. 36:374. PubMed.
Product Citations
  1. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  2. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  3. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  4. Alissafi T, et al. 2018. J Clin Invest. 128:3840. PubMed
  5. Bahmani B, et al. 2018. J Clin Invest. 128:4770. PubMed
  6. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  7. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  8. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  9. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  10. Sartorius R, et al. 2015. EMBO Mol Med. 7: 973 - 988. PubMed
  11. Kondo M, et al. 2016. J Immunol. 196: 563 - 572. PubMed
  12. Pizzolla A, et al. 2016. PLoS One. 11: 0160407. PubMed
  13. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  14. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
RRID
AB_1134184 (BioLegend Cat. No. 127807)
AB_1134183 (BioLegend Cat. No. 127808)

Antigen Details

Distribution

On a subset of T cells

Function
In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Interaction
Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor
TCR recognizes MHC/peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Kubo RT, et al. 1989. J. Immunol.. 142:2736.
2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.

Gene ID
21473 View all products for this Gene ID
UniProt
View information about TCR Valpha2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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