PerCP/Cyanine5.5 anti-mouse TCR Vα2 Antibody

Pricing & Availability
Clone
B20.1 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vα 2
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
B20dot1_PerCPCyanine55_TCR-Va2_Antibody_081618
C57BL/6 splenocytes were stained with CD3 APC and TCR Va2 (Clone B20.1) PerCP/Cyanine5.5 (left), or rat IgG2a, ? PerCP/Cyanine5.5 isotype control (right).
  • B20dot1_PerCPCyanine55_TCR-Va2_Antibody_081618
    C57BL/6 splenocytes were stained with CD3 APC and TCR Va2 (Clone B20.1) PerCP/Cyanine5.5 (left), or rat IgG2a, ? PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
127813 25 µg 84,00€
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127814 100 µg 220,00€
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Description

The TCR alpha (α) chain complexes with the TCR beta (β) chain to form the T cell receptor in 95% of T cells, whereas the remaining 5% of T cells express gamma and delta chains (γ/δ).  TCR Vα2 is a distinct TCR subfamily found in mice having the a, b, and c haplotypes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble TCR from mouse CTL clone KB5-C20
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The B20.1 antibody recognizes most members of the Va2 TCR subfamily in mice having the a, b, and c haplotypes.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Pircher H, et al. 1992. Eur. J. Immunol.. 22:399.
  2. Gregoire C, et al. 1991. P. Natl. Acad. Sci. USA 88:8077.
  3. Kao C, et al. 2005. Int. Immunol.17:1607. PubMed
  4. Steptoe RJ, et al. 2007. J. Immunol. 178:2094. PubMed
  5. Rao RR, et al. 2012. Immunity. 36:374. PubMed.
Product Citations
  1. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  2. Ooi JD, et al. 2017. Nature. 545:243. PubMed
  3. Caronni N, et al. 2018. Cancer Res. 78:1685. PubMed
  4. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
  5. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
RRID
AB_1186118 (BioLegend Cat. No. 127813)
AB_1186116 (BioLegend Cat. No. 127814)

Antigen Details

Distribution

On a subset of T cells

Function
In combination of V β, Vα recognizes MHC/peptide complex on the antigen-presenting cells.
Interaction
Associate with Vβ and CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor
TCR recognizes MHC/peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Kubo RT, et al. 1989. J. Immunol.. 142:2736.
2. Pircher H, et al. 1992. Eur. J. Immunol. 22:399.

Gene ID
21473 View all products for this Gene ID
UniProt
View information about TCR Valpha2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 08-16-2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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