Cell Staining Buffer

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Regulatory Status
RUO
Other Names
Staining Buffer, Washing Buffer
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420201 500 mL 72€
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Description

Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell suspensions. Cell Staining Buffer contains bovine calf serum as a protein carrier to reduce non-specific binding of antibodies and fluorochrome reagents to target cells. It also contains a metabolic inhibitor, sodium azide (NaN3), to inhibit patching and capping of cell surface antigens. To prevent interference in biotin/avidin indirect staining protocols, Cell Staining Buffer is formulated without biotin, calcium, magnesium, and EDTA.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
ICC, ICFC - Reported in the literature, not verified in house

Application References
  1. Koon HW, et al. 2013. J Vis exp. 68:4208. PubMed.
  2. Yu DH, et al. 2013. Mol Cell Biol. 33:1845. PubMed.
  3. Garcia JA, et al. 2013. J Immunol. 191:1063. PubMed
  4. Lukas S, et al. 2013. J Biomol Screen. 19:407. PubMed
  5. Murali VK, et al. 2014. J. Virol. 88:903. PubMed
  6. Kusner LL, et al. 2014. PLoS One. 9:102231. PubMed
  7. de Vires M, 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  8. Della-Torre E, et al. 2014. Ann Rheum Dis. PubMed
  9. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
Product Citations
  1. Lee HH, et al. 2021. Nat Commun. 12:2788. PubMed
  2. Su T, et al. 2021. Metabolites. 11:. PubMed
  3. Murthy S, et al. 2022. Cell Death Dis. 13:53. PubMed
  4. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  5. Oreskovic E, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  6. Lau D, et al. 2022. Nat Commun. 13:4053. PubMed
  7. Hanga MP, et al. 2021. Biotechnol Bioeng. 118:3175. PubMed
  8. Alber S, et al. 2022. Front Immunol. 13:838636. PubMed
  9. Mirlekar B, et al. 2020. Cancer Immunol Res. 0.536111111. PubMed
  10. Nuccitelli R, et al. 2017. J Immunother Cancer . 10.1186/s40425-017-0234-5. PubMed
  11. Djeungoue–Petga MA, et al. 2019. Cell Death Dis. 1.069444444. PubMed
  12. Markowicz-Piasecka M, et al. 2020. Apoptosis. 25:426. PubMed
  13. Yildirim Z, et al. 2022. EMBO Mol Med. 14:e15344. PubMed
  14. Albaghdadi AJH, et al. 2022. Int J Mol Sci. 23:. PubMed
  15. Kodani Y, et al. 2022. Front Endocrinol (Lausanne). 13:941166. PubMed
  16. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  17. Yoshida M, et al. 2022. Nature. 602:321. PubMed
  18. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  19. Stewart AN, et al. 2021. J Neuroinflammation. 18:113. PubMed
  20. Gawriluk TR, et al. 2020. Front Immunol. 1.635416667. PubMed
  21. Kusner L, et al. 2014. PLoS One. 9:102231. PubMed
  22. Lee H, et al. 2019. Cancer Res. 79:2839. PubMed
  23. Khayrullina G, et al. 2020. Skelet Muscle. 10:16. PubMed
  24. Swaroop M, et al. 2018. Hum Mol Genet. 27:3612. PubMed
  25. Mercatelli D, et al. 2021. Methods Protoc. 4: . PubMed
  26. Sima LE, et al. 2021. J Immunother Cancer. 9:. PubMed
  27. Yang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  28. Li Y, et al. 2021. Nat Commun. 12:87. PubMed
  29. Pino P, Cardona A 2011. J Vis Exp. 48: 2348. PubMed
  30. Cardona S, et al. 2015. ASN Neuro. 7: 1759091415608204. PubMed
  31. Karagiannis TT, et al. 2020. Nat Commun. 2.271527778. PubMed
  32. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  33. Michaud D, et al. 2022. Front Immunol. 12:745873. PubMed
  34. Brito-Pérez Y, et al. 2021. Immun Inflamm Dis. 9:1541. PubMed
  35. Mei KC, et al. 2020. ACS Nano. 14:13343. PubMed
  36. Nam AR, et al. 2020. Cancer Res Treat. 52:945. PubMed
  37. Deng W, et al. 2017. J Immunol. 198(1):492-504. PubMed
  38. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  39. Garcia J, et al. 2013. J Immunol. 191:1063. PubMed
  40. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  41. Gilad Y, et al. 2019. Sci Rep. 9:15099. PubMed
  42. Lim JA, et al. 2018. BMC Cancer. 0.860416667. PubMed
  43. Ji WJ, et al. 2017. Cytometry B Clin Cytom. 92:371. PubMed
  44. Wurzer H, et al. 2021. Front Immunol. 12:619069. PubMed
  45. Yoshioka H, et al. 2021. Front Cell Dev Biol. 9:621045. PubMed
  46. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  47. Ogbe A, et al. 2022. JCI Insight. . PubMed
  48. Song S, et al. 2022. Front Immunol. 13:804306. PubMed
  49. Drummer DJ, et al. 2022. Int J Exerc Sci. 15:686. PubMed
  50. Krivanek J, et al. 2020. Nat Commun. 3.802777778. PubMed
  51. Tang B, et al. 2020. Clin Cancer Res. 26:2216. PubMed
  52. Liao C, et al. 2017. Endocr Relat Cancer. 10.1530/ERC-16-0138. PubMed
  53. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  54. Luo G, et al. 2017. PLoS One. 12:e0187305. PubMed
  55. Moose DL, et al. 2020. Cell Rep. 30:3864. PubMed
  56. Soldi R, et al. 2020. PLoS One. 15:e0235705. PubMed
  57. Datta A, et al. 2022. J Fungi (Basel). 8:. PubMed
  58. Liu Y, et al. 2022. Nat Commun. 13:2665. PubMed
  59. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  60. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  61. Choreño-Parra JA, et al. 2021. J Immunol Res. 2021:5517856. PubMed
  62. Bodnar B, et al. 2021. Front Cell Neurosci. 15:682272. PubMed
  63. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  64. Wan J, et al. 2021. Front Pharmacol. 11:590723. PubMed
  65. Zhang X, et al. 2020. J Immunol. 205:1743. PubMed
  66. Vries M, et al. 2014. Am J Physiol Lung Cell Mol Physiol. 307:240. PubMed
  67. Yu D, et al. 2013. Mol Cell Biol. 33:1845. PubMed
  68. Fuentes I, et al. 2020. Sci Rep. 10:15064. PubMed
  69. Huangfu J, et al. 2021. Cell Death Dis. 12:386. PubMed
  70. Hao Q, et al. 2022. Front Immunol. 13:1011922. PubMed
  71. Lee D, et al. 2022. J Clin Invest. Online ahead of print. PubMed
  72. Wang D, et al. 2015. Gastroenterology. Available online 7 August 2015. PubMed
  73. Gorshkov K, et al. 2020. bioRxiv. . PubMed
  74. Feng S, et al. 2017. Cancer Sci. 108:12:00. PubMed
  75. Zhou L, et al. 2017. Tumour Biol. 39:1010428317694313. PubMed
  76. Hu G, et al. 2022. Cell Death Dis. 13:640. PubMed
  77. Yuan F, et al. 2022. Front Immunol. 13:798583. PubMed
  78. Rogers BM, et al. 2021. J Exp Med. 218:. PubMed
  79. Xie L, et al. 2020. Front Oncol. 1.354166667. PubMed
  80. Watkins–Schulz R, et al. 2019. Biomaterials. 205:94. PubMed
  81. Ryans K, et al. 2017. BMC Immunology . 10.1186/s12865-017-0212-1. PubMed
  82. Emmrich S, et al. 2021. Aging Cell. 20:e13477. PubMed
  83. Uprichard SL, et al. 2022. Viruses. 14:. PubMed
  84. Li X, et al. 2022. Bone Res. 10:46. PubMed
  85. Zhong W, et al. 2022. Nat Commun. 13:4390. PubMed
  86. Balan I, et al. 2022. Front Immunol. 13:940095. PubMed
  87. Lukas S, et al. 2013. J Biomol Screen. 19:407. PubMed
  88. Jia C, et al. 2015. Fish Shellfish Immunol. 45: 300-306. PubMed
  89. Garg A, Trout R, Spector S 2017. Sci Rep. 10.1038/srep44485. PubMed
  90. Teravskis PJ, et al. 2018. J Neurosci. 38:9754. PubMed
  91. Kodani Y, et al. 2022. Eneuro. 9:. PubMed
  92. Zhou H, et al. 2022. Oncoimmunology. 11:2057892. PubMed
  93. Han Y, et al. 2021. Cell Death Dis. 12:993. PubMed
  94. Pang K, et al. 2022. Cell Death Dis. 13:169. PubMed
  95. Shiraki N, et al. 2022. Stem Cell Reports. 17:221. PubMed
  96. McQueen BE, et al. 2020. Infect Immun. :88. PubMed
  97. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  98. Della-Torre E, et al. 2015. Ann Rheum Dis. 74: 2236 - 2243. PubMed
  99. Putz EJ, et al. 2019. J Dairy Sci. 102:9268. PubMed
  100. Wang Z, et al. 2020. Oncogenesis. 0.431944444. PubMed
  101. Crifò B, et al. 2019. J Immunol. 202:1521. PubMed
  102. La T, et al. 2018. Cancer Res. 78:6666. PubMed
  103. Bartkowiak K, et al. 2021. Cancers (Basel). 13: . PubMed
  104. Besnier M, et al. 2021. Antioxidants (Basel). 10:. PubMed
  105. Liu X, et al. 2022. Cell Discov. 8:58. PubMed
  106. Toutounchian J, et al. 2016. Mol Pharmacol. 91(1):1-13. PubMed
  107. Verma N, et al. 2020. Sci Adv. 6:00. PubMed
  108. Chen CZ, et al. 2020. ACS Pharmacol Transl Sci. 1165:3. PubMed
  109. Matsumoto K, et al. 2017. PLoS One. 12(6):e0175406. PubMed
  110. Surdziel E, et al. 2017. PLoS One.. 10.1371/journal.pone.0183679. PubMed
  111. Li Y, et al. 2019. Mol Med Rep. 3.90625. PubMed
  112. Putz EJ, et al. 2020. BMC Vet Res. 0.9. PubMed
  113. Hernandez-Davies JE, et al. 2022. Sci Rep. 12:9198. PubMed
  114. McQueen BE, et al. 2021. Infect Immun. 89:e0007221. PubMed
  115. Nadeu F, et al. 2022. Nat Med. 28:1662. PubMed
  116. Gupta Y, et al. 2021. Int J Biol Macromol. 183:203. PubMed
  117. Jordakieva G, et al. 2021. Oncoimmunology. 10:1880687. PubMed
  118. Chen DL, et al. 2021. Mol Cancer. 20:166. PubMed
  119. Becerril C, et al. 2021. Biomolecules. 11:. PubMed
  120. Ishida M, et al. 2021. Int J Mol Sci. 22:. PubMed
  121. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  122. Li Y, et al. 2021. Stem Cell Res Ther. 12:358. PubMed
  123. Koikawa K, et al. 2021. Cell. 184:4753. PubMed
  124. Mitry MA, et al. 2020. American Journal of Physiology-Cell Physiology. 318(2):C380-C391. PubMed
  125. Dai S, et al. 2017. Autophagy. 1.538194444. PubMed
  126. Alasady MJ, et al. 2021. PLoS One. 16:e0256238. PubMed
  127. Ren C, et al. 2022. J Cell Mol Med. 26:1071. PubMed
  128. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  129. Thiele M, et al. 2015. J Immunol. 195: 2343-2352. PubMed
  130. Gorshkov K, et al. 2020. ACS Infect Dis. . PubMed
  131. Ye Y, et al. 2017. Sci Immunol. 2:17. PubMed
  132. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  133. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  134. Yang WH, et al. 2018. Cancer Res. 78:3761. PubMed
  135. Tajima M, et al. 2022. Curr Protoc. 2:e540. PubMed
  136. Hasanpourghadi M, et al. 2021. Curr Trends Microbiol. 15:1. PubMed
  137. Mirlekar B, et al. 2022. Cell Rep Med. :100744. PubMed
  138. Han MG, et al. 2022. Cancers (Basel). 14:. PubMed
  139. Dustin CM, et al. 2021. J Immunol. 206:2989. PubMed
  140. Xu M, et al. 2022. SLAS Discov. 27:86. PubMed
  141. Babaei A, et al. 2020. Daru. 28:555. PubMed
  142. Pearce AC, et al. 2021. J Biol Chem. 297:100928. PubMed
  143. Wang Y, et al. 2020. Virol Sin. 36:122. PubMed
  144. Harro CM, et al. 2020. J Clin Invest. . PubMed
  145. Gutierrez WR, et al. 2021. Sci Rep. 1.220833333. PubMed
  146. Fries-Craft K, et al. 2020. PLoS One. 15:e0236106. PubMed
  147. Khoa LTP, et al. 2020. STAR Protoc. 1:100136. PubMed
  148. Motedayyen H, et al. 2017. BMC Res Notes. . 10.1186/s13104-017-2880-6. PubMed
  149. Aguisanda F, et al. 2017. Orphanet J Rare Dis. 10.1186/s13023-017-0670-9. PubMed
  150. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  151. You C, et al. 2021. Cancers (Basel). 13:. PubMed
  152. Salameh JW, et al. 2022. Bioengineering (Basel). 9:. PubMed
  153. Luque-Martin R, et al. 2021. J Immunol. 207:555. PubMed
  154. Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
  155. King P, et al. 2021. Int J Oncol. 59:. PubMed
  156. Peng Z, et al. 2022. J Neuroinflammation. 19:186. PubMed
  157. Hwang B, et al. 2021. Nat Methods. 18:903. PubMed
  158. Chen H, et al. 2022. Mol Ther Methods Clin Dev. 27:431. PubMed
  159. Ma H, et al. 2015. Sci Rep. 5: 15121. PubMed
  160. Park SR, et al. 2020. Cell Rep. 32:108077. PubMed
  161. Gorshkov K, et al. 2020. J Pharmacol Exp Ther. 374:500. PubMed
  162. Tak YE, et al. 2017. Nat Methods. 14:1163. PubMed
  163. Li B, et al. 2018. Cancer Res. 78:3938. PubMed
  164. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  165. Ji Y, et al. 2021. Nanomaterials (Basel). 11: . PubMed
  166. Peng X, et al. 2021. Stem Cell Res Ther. 198:12. PubMed
  167. Chen Q, et al. 2020. Aging (Albany NY). 12:3682. PubMed
  168. Koury J, et al. 2019. PLoS One. 14:e0218670. PubMed
  169. Genge PC, et al. 2021. STAR Protoc. 2:100900. PubMed
  170. Yang Zhou J, et al. 2022. Front Immunol. 13:898473. PubMed
  171. Li B, et al. 2021. Clin Cancer Res. 27:6543. PubMed
  172. Gao M, et al. 2022. BMC Mol Cell Biol. 23:32. PubMed
  173. Hsiao W, et al. 2015. PLoS One. 10: 0145880. PubMed
  174. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  175. Wang M, et al. 2016. Sci Rep. 6: 34060. PubMed
  176. Shi X, et al. 2017. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 10.1016/j.bbamcr.2017.05.012. PubMed
  177. Brocato ER, et al. 2018. PLoS One. 13:e0197701. PubMed
  178. Nozhat Z, et al. 2018. Daru. 26:93. PubMed
  179. Tao Z, et al. 2022. Cells. 11:. PubMed
  180. Fan L, et al. 2022. Theranostics. 12:6809. PubMed
  181. Saddawi-Konefka R, et al. 2022. Nat Commun. 13:4298. PubMed
  182. Arora S, et al. 2021. Med (N Y). 2:938. PubMed
  183. Chan M, et al. 2021. Mol Syst Biol. 17:e10426. PubMed
  184. Seo HR, et al. 2021. Cancer Res Treat. Online ahead of print. PubMed
  185. Wang L, et al. 2021. Metabolites. 11:. PubMed
  186. Chen X, et al. 2021. Cell Death Dis. 12:1123. PubMed
  187. Pogozhykh D, et al. 2017. Stem Cell Res Ther. 10.1186/s13287-017-0512-7. PubMed
  188. Mishra P, et al. 2016. PLoS Negl Trop Dis. 10: 0004787. PubMed
  189. Williams K, et al. 2015. J Neuroimmunol. 285: 76-93. PubMed
  190. Simic D, et al. 2019. Exp Hematol. 72:36. PubMed
  191. Di Franco S, et al. 2021. STAR Protoc. 2:100880. PubMed
  192. Wang X, et al. 2021. Sci Transl Med. 13:. PubMed
  193. Chen C, et al. 2022. iScience. 25:103645. PubMed
  194. Yang P, et al. 2022. Oxid Med Cell Longev. 2021:5826932. PubMed
  195. Xiao C, et al. 2021. STAR Protoc. 2:100789. PubMed
  196. Bianchi A, et al. 2022. Genome Biol. 23:229. PubMed
  197. Murali V, et al. 2014. J Virol. 88:903. PubMed
  198. Palma M, et al. 2017. Haematologica. 10.3324/haematol.2016.151100. PubMed
  199. Dudley A, et al. 2017. American Journal of Veterinary Research. 10.2460/ajvr.78.5.589. PubMed
  200. Kawamura D, et al. 2017. Oncol Rep.. 10.3892/or.2017.5974. PubMed
  201. Quan M, et al. 2019. Oncol Rep. 41:3051. PubMed
  202. Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
  203. Künzel K, et al. 2022. Heliyon. 8:e10365. PubMed
  204. Kaya-Tilki E, et al. 2021. PLoS One. 16:e0260633. PubMed
  205. Samuel RM, et al. 2020. Cell Stem Cell. 27:876. PubMed
  206. Zhou Q, et al. 2016. Sci Rep. 6:39071. PubMed
  207. Kaabinejadian S, et al. 2016. J Immunol. 196: 4263 - 4273. PubMed
  208. Yu W, et al. 2015. Cell Rep. 12: 610- 21. PubMed
  209. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
  210. Zeng Y, et al. 2019. FASEB J. 33:6596. PubMed

Antigen Details

Cell Type
Tregs
Gene ID
NA

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Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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