- BVD3-1F9 (See other available formats)
- Other Names
- Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
- Rat IgG1, κ
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- Product Citations
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IL-3 is a highly species-specific pleiotropic factor produced primarily by activated T cells though also by mast cells, keratinocytes, and astrocytes, which stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. The BVD3-1F9 antibody can neutralize the bioactivity of natural or recombinant IL-3.Product Details
- Antibody Type
- Host Species
- Yeast-expressed, recombinant human IL-3
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection, ELISPOT Detection
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-3 protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA1-3,6 or ELISPOT4 Detection: The biotinylated BVD3-1F9 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified BVD8-3G11 (Cat. No. 500502) antibody as the capture antibody.
Flow Cytometry: The fluorochrome-labelled BVD3-1F9 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-3 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Additional reported applications (for the relevant formats) include: immunoprecipitation6, Western blotting6, neutralization1,6, immunohistochemical staining5,7 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
- Application References
- Abrams J, et al. 1992. Immunological Reviews 127:5.
- Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.
- Abrams J. 1995. Curr. Prot. Immunol.. 6.20.
- Mahanty S, et al. 1992. J. Immunol. 148:3567.
- Andersson U, et al. 1993. Detection and quantification of gene expression. New York: Springer-Verlag.
- Kaushansky K. 1992. J. Clin. Invest. 90:1879.
- Andersson J, et al. 1994. Immunology 83:16.
AB_2123713 (BioLegend Cat. No. 500604)
- Cytokine; 14-30 kD (Mammalian)
- Proliferation/differentiation of almost all types of hematopoietic progenitors into granulocytes, macrophages, erythroid cells, megakaryocytes, mast cell colonies; induces expression of 20-α-steroid dehydrogenase, histidine, ornithine decarboxylase
- Cell Sources
- Activated T cells, mast cells, eosinophils, keratinocytes, NK cells, endothelial cells
- Cell Targets
- Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
- IL-3R, α subunit (CD123), common β subunit (CDw131)
- Biology Area
- Cell Biology, Stem Cells
- Molecular Family
- Growth Factors, Cytokines/Chemokines
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Frendl G. 1992. Int. J. Immunopharmacol. 14:421.
3. Ihle J. 1992. Chem. Immunology 51:65.
- Upregulated by T cell stimulation; downregulated by glucocorticoids
- Gene ID
- 3562 View all products for this Gene ID
- View information about IL-3 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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