APC/Cyanine7 anti-mouse CD117 (c-kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_APCCyanine7_CD117_Antibody_022519
C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (c-kit) (clone 2B8) APC/Cyanine7 (left) or rat IgG2b, ? APC/Cyanine7 isotype control (right).
  • 2B8_APCCyanine7_CD117_Antibody_022519
    C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (c-kit) (clone 2B8) APC/Cyanine7 (left) or rat IgG2b, ? APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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105825 25 µg 76€
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105826 100 µg 184€
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  3. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  4. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
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  6. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  7. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
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  9. Castiello MC, et al. 2020. J Allergy Clin Immunol. . PubMed
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  11. Iturri L, et al. 2021. Immunity. . PubMed
  12. Wu X, et al. 2016. Nat Commun. 7:10533. PubMed
  13. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  14. Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed
  15. Li Z, et al. 2022. Elife. 11:. PubMed
  16. Taylor D, et al. 2011. J Immunol. 186:5956. PubMed
  17. Nguyen DTT, et al. 2020. Nat Commun. 11:2026. PubMed
  18. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  19. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  20. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  21. Li Y, et al. 2021. Nat Commun. 0.843055556. PubMed
  22. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  23. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  24. Nurkhametova D, et al. 2019. Front Cell Neurosci. 13:45. PubMed
  25. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  26. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  27. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  28. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  29. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
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  33. Bell CC, et al. 2019. Nat Commun. 10:2723. PubMed
  34. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  35. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
  36. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  37. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  38. Comoglio F, et al. 2018. Genome Res. 10.1101/gr.227272.117. PubMed
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  46. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
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  48. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  49. Mana MD, et al. 2021. Cell Reports. 35(10):109212. PubMed
  50. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  51. Abdala-Valencia H, et al. 2015. J Immunol. 195: 1377-1387. PubMed
  52. Rüder C, et al. 2014. PLoS One. 9:104644. PubMed
  53. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  54. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  55. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  56. Bikorimana E, et al. 2014. J Vis Exp. 92: 52022. PubMed
  57. Stine RR, et al. 2020. Cell Stem Cell. 25(6):830-845. PubMed
  58. Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed
  59. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  60. Koide S, et al. 2022. iScience. 25:103603. PubMed
  61. Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed
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  63. Krivtsov AV, et al. 2020. Cancer Cell. 36(6):660-673.e11.. PubMed
  64. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  65. Lee SG, et al. 2020. J Nucl Med. . PubMed
  66. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
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  71. Li X, et al. 2019. Stem Cells. 37:937. PubMed
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  78. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
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  81. Young K, et al. 2021. Cell Stem Cell. . PubMed
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RRID
AB_1626280 (BioLegend Cat. No. 105825)
AB_1626278 (BioLegend Cat. No. 105826)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02-25-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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