- AR1 (See other available formats)
- Other Names
- IRF3, IRF-3, interferon regulatory factor 3
- Mouse IgG1, κ
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- Product Citations
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IRF-3 is a roughly 50 kD member of the interferon regulatory transcription factor (IRF) family. Along with other members of the IRF family IRF-3 plays a key role in the innate immune response to viral infection. It is located ubiquitously in an inactivated form in the cytoplasm of many immune cells. When phosphorylated it forms a complex with CREBBP to upregulate many immune responses integral to innate immune function.Product Details
- Antibody Type
- Host Species
- Recombinant human IRF-3 protein (entire protein)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Red Laser (633 nm)
- Application References
- Rustagi A, et al. 2012. Methods. 59(2):225-32
AB_2888751 (BioLegend Cat. No. 395905)
AB_2888751 (BioLegend Cat. No. 395906)
- Human IRF-3 has 427 amino acids with predicted Mw 47.2 kD and observed Mw 52 kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9 kD and observed Mw 45 kD.
Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.
- CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
- Pattwell SS, et al. 2017. Trends Mol Med. (9):773-776
- Zhang ZX, et al. 2017. Leuk Lymphoma. 58(3): 646-654
- Zhao B, et al. 2016. Proc Natl Acad Sci USA. 113(24)E3403-12
- Gene ID
- 3661 View all products for this Gene ID
- View information about IRF3 on UniProt.org
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