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Western-Ready™ Protein Sample Loading Buffer (5X)

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426311 20 mL 38€
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Description

Western-Ready™ Protein Sample Loading Buffer (5X) can be used for the preparation of samples for non-reducing and reducing SDS-PAGE.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
300 mM Tris-HCl pH 6.8, 10% Sodium dodecyl sulfate, 30% glycerol, 0.02% Bromophenol blue
Storage & Handling
Ambient room temperature
Application

WB - Quality tested
Recommended Usage

Dilute Western-Ready™ Protein Sample Loading Buffer (5X) to a 1X concentration (1:4 by volume) using appropriate amount of sample and diluting solution (ie: water, lysis buffer, etc). For sample that are to be used for reducing PAGE, a reducing agent such as as β-ME, DTT, or TCEP must be added to buffer prior to mixing and heating sample.

Application Notes

Western-Ready™ Protein Sample Loading Buffer (5X) contains SDS, which may precipitate at cooler temperatures. If preceipitates are observed in the buffer, heat in 37°C water bath for 5 min to bring SDS back into solution.

Western-Ready™ Protein Sample Loading Buffer (5X) is compatible with Bis-Tris, Tris-Glycine, and Tris-Acetate gels.

Western-Ready™ Protein Sample Loading Buffer (5X) does not include any reducing agents. For reducing SDS-PAGE, we recommend adding a reducing agent such as ß-ME, DTT, TCEP to buffer prior to mixing with sample.

This buffer should not be used for native PAGE.

This buffer may be used to elute captured immune complexes during immunoprecipitation-based applications.

Antigen Details

Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 11/19/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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