Purified anti-Lysozyme Antibody

Pricing & Availability
Clone
Poly28600 (See other available formats)
Regulatory Status
RUO
Other Names
Lysozyme, LZM, 1,4-Beta-N-Acetylmuramidase C, EC 3.2.1.17, Lysozyme C, Lysozyme (Renal Amyloidosis) 2
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly28600_Purified_Lysozyme_Antibody_1_051519.png
Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 20 µg of human spleen lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28600_Purified_Lysozyme_Antibody_1_051519.png
    Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 20 µg of human spleen lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28600_Purified_Lysozyme_Antibody_2_051519.png
    Western blot of purified anti-Lysozyme antibody (Poly28600). Lane 1: Molecular weight marker; Lane 2: 10 µg of rat kidney lysate; Lane 3: 20 µg of mouse kidney lysate. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28600_Purified_Lysozyme_Antibody_3_051519.png
    IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded human spleen tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28600_Purified_Lysozyme_Antibody_4_051519.png
    IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • Poly28600_Purified_Lysozyme_Antibody_5_051519.png
    IHC staining of purified anti-Lysozyme antibody (Poly28600) on formalin-fixed paraffin-embedded rat kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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860001 25 µg 100€
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Description

Lysozyme is an antimicrobial enzyme  that is abundantly present in secretions such as tears, saliva and mucus. Its hydrolase activity leads to the breakdown of the gram-positive bacterial cell wall and lysis of the bacteria.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant Lysozyme expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml for human, and 1.0 - 2.0 µg per ml for mouse and rat. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810736 (BioLegend Cat. No. 860001)

Antigen Details

Structure
Lysozyme is a 148 amino acid protein with an apparent molecular mass of ~ 16kD.
Distribution

Tissue Distribution: Spleen, kidney, milk, tears, and plasma
Cellular Distribution: Cytosol

Function
Lysozyme is expressed in many immune cells including monocytes and macrophages. It plays an important role in innate immunity by hydrolyzing peptidoglycans found in bacterial cell wall and thus destroying the bacteria.
Interaction
Bacterial peptidoglycan
Cell Type
Macrophages, Mesenchymal cells, Monocytes, Neutrophils
Biology Area
Bacterial proteins and Toxins, Cell Biology, Immunology, Innate Immunity
Molecular Family
Enzymes and Regulators, Innate Immune Signaling, Proteases
Antigen References
  1. Mallamace D, et al. 2018. Int J Mol Sci. 19(12): E3825.
  2. Delorme-Axford E and Klionsky DJ. 2018. Autophagy. 14(3):365.
Gene ID
4068 View all products for this Gene ID 17110 View all products for this Gene ID 25211 View all products for this Gene ID
UniProt
View information about Lysozyme on UniProt.org
Go To Top Version: 2    Revision Date: 05/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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