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Ultra-LEAF™ Purified anti-human CD272 (BTLA) Antibody

Pricing & Availability
Clone
W21076A (See other available formats)
Regulatory Status
RUO
Other Names
CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W21076A_ULEAF_CD272_Block_090823
Biotinylated recombinant human BTLA-Fc chimera (Cat. No. 794904) (black circles) binds to immobilized recombinant mouse HVEM-Fc chimera (Cat. No. 771304). Ultra-LEAF™ purified anti-human CD272 (BTLA) (clone W21076A) (purple squares) inhibits this binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have this effect. ND50 range: 4-30 ng/mL
  • W21076A_ULEAF_CD272_Block_090823
    Biotinylated recombinant human BTLA-Fc chimera (Cat. No. 794904) (black circles) binds to immobilized recombinant mouse HVEM-Fc chimera (Cat. No. 771304). Ultra-LEAF™ purified anti-human CD272 (BTLA) (clone W21076A) (purple squares) inhibits this binding in a dose-dependent manner, whereas the Ultra-LEAF™ purified rat IgG2b, κ isotype control (Cat. No. 400644) (gray triangles) does not have this effect. ND50 range: 4-30 ng/mL
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622053 100 µg 276€
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622054 1 mg 964€
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Description

BTLA (B and T lymphocyte attenuator, CD272) is a type I transmembrane glycoprotein with four extracellular cysteine-rich domains. It is an inhibitory molecule structurally and functionally similar to cytotoxic T lymphocyte antigen-4 (CTLA-4) and programmed death-1 (PD-1). These molecules belong to the immunoglobulin superfamily that binds B7 family members. Different from CTLA-4 and PD-1 binding with the B7 family members, BTLA interacts with herpes virus entry mediator (HVEM/TNFRSF14) which belongs to TNFR family. BTLA/HVEM interaction was the first demonstration of crosstalk between these two family members. BTLA/HVEM interaction limits T cell activity in vivo and regulates the homeostatic expansion of CD4+ and CD8+ T cells. BTLA/HVEM interaction negatively modulates T cell activation. HVEM induces BTLA tyrosine phosphorylation and inhibits T-cell proliferation. There is accumulating evidence suggesting that BTLA/HVEM signaling also plays an important role in autoimmunity and infection at mucosal surfaces. The BTLA/HVEM interaction does not prevent the simultaneous binding of other HVEM ligands such as LIGHT/TNFSF14 or Lymphotoxin-alpha. BTLA is expressed on T cells, B cells, macrophages, dendritic cells, and natural killer cells. Constitutive expression level of BTLA on resting T cells are very low, but increase after the activation of T cells. BTLA is highly expressed on resting B cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD272 (BTLA)
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 0.007 µg/mL biotinylated recombinant human BTLA-Fc chimera to 0.05 µg/mL immobilized recombinant mouse HVEM-Fc chimera. ND50 range: 4-30 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Disulfide bond-linked homodimer
Distribution

Expressed in activated B, T (activated, memory), Th1, Th2 and Th17 Effector T-cells of patients with systemic lupus erythematosus, dendritic cells, macrophages, and hematopoietic stem cells

Function
B cell co-stimulation, proliferation and Ig production, limits T cell activity in vivo and homeostatic expansion of CD4+ and CD8+ T cells, NKT differentiation
Interaction
Lymphocytes
Ligand/Receptor
HVEM (TNFRSF14)
Cell Type
B cells, Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References
  1. Gavrieli M, et al. 2003. Biochem. Biophys Res Commun. 312:1236-43.
  2. Gonzalez LC, et al. 2005. Proc Natl Acad. Sci. USA. 102:1116.
  3. Sedy JR, et al. 2005. Nat Immunol. 6:90-8.
  4. Kronenberg KM, et al. 2009. Proc Natl Acad Sci USA. 106:6244.
  5. Shui JW, et al. 2011. J Leukoc Biol. 89:517-23.
  6. Ofter C, et al. 2019. Int J Mol Sci. 20:4505.
  7. Yu X, et al. 2019. Front Immunol. 10: 617.
Gene ID
151888 View all products for this Gene ID
UniProt
View information about CD272 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/08/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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