Recombinant Rat CCL5 (RANTES) (carrier-free)

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Other Names
RANTES, Scya5
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Rat_CCL5_Rantes_RECOM_CF_040717
Rat CCL5 chemoattracts Baf3-hCCR5 transfectants in a dose dependent manner.
  • Rat_CCL5_Rantes_RECOM_CF_040717
    Rat CCL5 chemoattracts Baf3-hCCR5 transfectants in a dose dependent manner.
Cat # Size Price Quantity Avail. Save
763902 10 µg 68€
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763904 25 µg 160€
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763906 100 µg 472€
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Description

CCL5 is a chemokine originally identified as a T-cell-specific gene. It directs the migration of leukocytes in immune and inflammatory responses. Increased CCL5 expression is associated with a wide range of inflammatory disorders and pathologies. This cytokine is linked as well with the induction or promotion of cancer (prostate and breast). In the latter situation, CCL5 can be angiogenic and also increase the metastatic potential of cancer cells by encouraging cellular migration to sites of its production. CCL5 also inhibits HIV-1 entry and infection by competing with the virus for CCR5 binding. Also, CCL5 participates in CNS homeostasis, brain inflammation, and ocular, and neurodegenerative diseases. Elevated plasma CCL5 and CCL2 were observed in autism spectrum disorder. Also, CCL5 and its receptors CCR1, CCR3 and CCR5 have also been observed in different pain models. CCL5 is expressed during spinal glial cell activation in a neuropathic pain model induced by chronic constriction injury surgery.

Product Details
Technical Data Sheet (pdf)

Product Details

Molecular Mass
The 68 amino acid recombinant protein has a predicted molecular mass of approximately 7.8 kD. The DTT-reduced protein migrates at approximately 10 kD and the non-reduced protein migrates at approximately 12 kD by SDS-PAGE. The N-terminal amino acid is Serine.
Purity
>95% as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution in 0.15 M NaCl, 0.1 M glycine pH 3.0.
Endotoxin Level
Less than 0.01 ng/per µg cytokine (0.1 EU/µg) as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Bioactivity was measured by its property to chemoattract Baf3-hCCR5 transfectants in a dose dependent manner. The ED50 is 0.6 - 3 ng/ml.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Chemokine
Distribution

Secreted by lymphocytes, macrophages, eosinophils, platelets, fibroblasts, microglia, astrocytes, and neurons. Expressed by hepatocytes, sinusoidal endothelial cells and biliary epithelium during chronic hepatitis C.

Function
CCL5 modulates the LPS-induced IL-6 and TNFα in peripheral blood monocytes; this modulation is through the induction of IL-10 by CCL5.
Interaction
CCL5 is chemoattractant for memory and activated T cells, monocytes, dendritic cells, eosinophils, NK cells, mast cells, and basophils.
Ligand/Receptor
CCL5 binds to CCR1, CCR3, CCR5, and GPR75 receptors. In addition CCL5 binds to CCR9; nevertheless, it does not induce calcium flux and therefore no chemotactic response.
Bioactivity
Rat CCL5 chemoattracts CCR5 expressing cells.
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Schall, et al. 1990. Nature 347:669.
2. Cocchi F, et al. 1995. Science 270:1811.
3. Shahrara S, et al. 2006. J. Immunology 177:5077.
4. Ignatov A, et al., 2006. Br J Pharmacol. 149:490.
5. Larrubia JR, et al. 2008. World J. Gastroenterol 14:7149.
6. Gleissner CA, et al. 2008. Arterioscler Thromb. Vasc. Biol. 28:1920.
7. Yin Q, et al. 2015. PLoS One. 10:e0115120.
8. Han YM, et al. 2017. Front Immunol. 8:11.

Gene ID
81780 View all products for this Gene ID
UniProt
View information about CCL5 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 0    Revision Date: 04/07/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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