Recombinant Human B7-2/CD86-Fc Chimera (carrier-free)

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Other Names
CD86, B7-2, T lymphocyte activation antigen CD86, Activation B7-2 antigen, CD28 antigen ligand 2, CD28LG2B7-2 antigen, B70, BU63, CTLA-4 counter-receptor B7.2, FUN-1
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Human_B7-2_CD86_RECOM_1_082118
Immobilized recombinant human B7-2/CD86-Fc binds biotinylated human CTLA-4 in a dose dependent manner with EC50 of 10 - 40 ng/mL.
  • Human_B7-2_CD86_RECOM_1_082118
    Immobilized recombinant human B7-2/CD86-Fc binds biotinylated human CTLA-4 in a dose dependent manner with EC50 of 10 - 40 ng/mL.
  • Human_B7-2_CD86_RECOM_2_082118
    Stability testing for Human B7-2/CD86-Fc. Human B7-2/CD86-Fc was aliquoted in PBS, pH 7.4 at 0.2 mg/mL. One aliquot was freeze-thawed four times (4x freeze/thaws), and compared to a control kept at 4°C (control). The samples were tested for their ability to bind biotinylated human CTLA-4.
Cat # Size Price Quantity Avail. Save
775602 10 µg 48€
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775604 25 µg 72€
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775606 100 µg 152€
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775608 500 µg 440€
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Description

CD86, also known as B7-2, is a member of the cell surface immunoglobulin superfamily. B7 family members are transmembrane molecules that exist predominantly as monomers. They are classified into three groups according to their functions. Group I members include B7-1, B7-2, and B7-H2. These members are either co-stimulatory or co-inhibitory in modulating T cell response. Group II members include B7-H1 (PD-L1) and B7-DC (PD-L2) which are ligands for PD-1. These members are involved in regulating induction and maintenance of peripheral immune tolerance. Group III members include B7-H3 and B7-H4 whose role in T cell regulation has yet to be defined. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Source
Human B7-2/CD86, amino acid Leu20-His245 (accession # P42081) with a C-terminal human IgG1 (Pro100-Lys330) and 6x His tag expressed in 293E cells.
Molecular Mass
The 465 amino acid recombinant protein has a predicted molecular mass of approximately 52.9 kD. The DTT-reduced protein migrates at approximately 70 kD and non-reduced protein migrates at approximately 140 kD by SDS-PAGE. The predicted N-terminal amino acid is Leu.
Purity
>95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in 1x PBS.
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for one month, at -20°C for six months, or at -70°C for one year. For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 0.2 - 1% BSA or HSA and stored in working aliquots at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
Activity
When human B7-2/CD86-Fc is immobilized at 0.5 µg/mL (100 µL/ well), biotinylated human CTLA-4 binds with EC50 of 10 - 40 ng/mL in a functional ELISA.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Immunoglobulin family
Distribution

Activated antigen presenting cells, including B cells, dendritic cells and macrophages

Function
B7-2 is a costimulatory molecule that is required for T cell proliferation and IL-2 production.
Interaction
Leukocytes, endothelial cells
Ligand/Receptor
CD28, CTLA4
Bioactivity
Measured by the ability of immobilized protein to bind biotinylated human CTLA-4
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors, Soluble Receptors
Antigen References
  1. Freeman GJ, et al. 1993. Science 262:909.
  2. Chen C, et al. 1994. J Immunol. 152:4929.
  3. Lanier LL, et al. 1995. J Immunol. 154:97.
  4. Carreno BM, et al. 2002. Annu Rev Immunol. 20:29.
  5. Rudd CE, et al. 2009. Immunol Rev. 229:12.
  6. Wing K, et al. 2011. Trends Immunol. 32:428.
Gene ID
942 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 1    Revision Date: 08/22/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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