Purified anti-RAG2 Antibody

Pricing & Availability
3G4A26 (See other available formats)
Other Names
V(D)J recombination-activating protein 2
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
Western blot analysis of PBMC (lane 1), mouse thymus (lane 2) and Hela (lane 3) cells using anti-RAG2 antibody (3G4A26). GAPDH antibody (poly6314) was used as loading control.
  • 3G4A26_RAG2_Purified_Antibody_WB_012114.jpg
    Western blot analysis of PBMC (lane 1), mouse thymus (lane 2) and Hela (lane 3) cells using anti-RAG2 antibody (3G4A26). GAPDH antibody (poly6314) was used as loading control.
Cat # Size Price Quantity Avail. Save
654902 100 µg 196€
Check Availability

Need larger quantities of this item?
Request Bulk Quote

During T cell and B cell development, RAG2 works together with RAG1 to induce DNA rearrangement and assembly of antigen receptor coding segments. Defects in RAG2 gene lead to the complete lack of mature T and B lymphocytes and result in severe combined immunodeficiency (SCID). RAG2 also functions in chromosome binding through its unique plant homeodomain (PHD). Sequestration of recombination signal sequences (RSS) within the nucleosome by RAG2 prevents access by the recombinase complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, Mouse
Antibody Type
Host Species
Partial RAG2 recombinant protein (70-516 aa).
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.05% sodium azide.
Affinity purified.
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-2.5 µg/ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application

AB_2561891 (BioLegend Cat. No. 654902)

Antigen Details

527 amino acids, predicted molecular weight of 59 kD. Containing a core RAG domain with DNA cleavage activity and an atypical PHD domain responsible for binding of trimethylated histone H3 (H3K4me3) and phospholipids.
RAG2 protein acts as a core subunit of the RAG complex, which is responsible for V(D)J recombination of T-cell receptors and immunoglobulins.
Interacts with RAG1, HMG1, and HMG2 in V(D)J recombination protein-DNA complexes.
Cell Type
B cells, Tregs
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Neuroscience
Molecular Family
Antigen References

1. Fugmann SD, et al. 2000. Annu. Rev. Immunol. 18:495.
2. Oettinger MA. 1992. Trends Genet. 8:413.
3. Hillion S, et al. 2009. Autoimmun. Rev. 8:599.
4. Deriano L, et al. 2011. Nature 471:119.
5. van Til NP, et al. 2012. Mol Ther. 20:1968.
6. Jones JM and Simkus C, et al. 2009. Arch. Immunol. Ther. Exp. 57:105.

Gene ID
5897 View all products for this Gene ID
View information about RAG2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/21/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Insert Note Here
Save Close Clear
Lab Timer
Login / Register
Forgot your password? Reset password?
Create an Account