- FUN-2 (See other available formats)
- Regulatory Status
- VI B051
- Other Names
- FCR II, FcγRII
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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CD32 is a 40 kD polymorphic transmembrane glycoprotein also known as FcγRII and FCRII. It is an immunoglobulin superfamily member expressed on monocytes/macrophages, granulocytes, platelets and B cells. There are at least 6 isoforms of CD32 resulting from alternative mRNA splicing. CD32 mediates phagocytosis and oxidative burst in granulocytes, as well as platelet aggregation and immunomodulation. The extracellular domain of CD32 binds to polymeric and aggregated IgG and immune complexes, while the intracellular domain has been reported to associate with SHP-1 (B1 isoform).Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of acetone-fixed frozen tissue sections.
Clone FUN-2 recognizes both CD32A and CD32B.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
- Product Citations
AB_2616924 (BioLegend Cat. No. 303215)
AB_2616925 (BioLegend Cat. No. 303216)
- Ig superfamily, polymorphic transmembrane glycoprotein, 40 kD
Monocytes, granulocytes, B cells, platelets
- Endocytosis, cytotoxicity, platelet aggregation, B cell function
- Aggregated IgG, immune complex
- Cell Type
- B cells, Dendritic cells, Granulocytes, Monocytes, Platelets
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
1. Stuart S, et al. 1989. EMBO J. 8:3657.
2. Huang Y, et al. 1999. Scand. J. Immunol. 49:177.
3. Hisaka H, et al. 1999. Pathobiology 67:92.
- Gene ID
- 2212 View all products for this Gene ID
- View information about CD32 on UniProt.org
- Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|APC anti-human CD32||FUN-2||FC|
|FITC anti-human CD32||FUN-2||FC|
|PE anti-human CD32||FUN-2||FC|
|Purified anti-human CD32||FUN-2||FC, IHC-F|
|Alexa Fluor® 647 anti-human CD32||FUN-2||FC|
|PE/Cyanine7 anti-human CD32||FUN-2||FC|
|PerCP/Cyanine5.5 anti-human CD32||FUN-2||FC|
|PE/Dazzle™ 594 anti-human CD32||FUN-2||FC|
|APC/Fire™ 750 anti-human CD32||FUN-2||FC|
|TotalSeq™-A0142 anti-human CD32||FUN-2||PG|
|TotalSeq™-C0142 anti-human CD32||FUN-2||PG|
|TotalSeq™-B0142 anti-human CD32||FUN-2||PG|
|APC/Cyanine7 anti-human CD32||FUN-2||FC|
|Biotin anti-human CD32||FUN-2||FC|
|Spark Violet™ 500 anti-human CD32||FUN-2||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC anti-human CD32
FITC anti-human CD32
PE anti-human CD32
Purified anti-human CD32
Alexa Fluor® 647 anti-human CD32
PE/Cyanine7 anti-human CD32
PerCP/Cyanine5.5 anti-human CD32
PE/Dazzle™ 594 anti-human CD32
APC/Fire™ 750 anti-human CD32
TotalSeq™-A0142 anti-human CD32
TotalSeq™-C0142 anti-human CD32
TotalSeq™-B0142 anti-human CD32
APC/Cyanine7 anti-human CD32
Biotin anti-human CD32
Spark Violet™ 500 anti-human CD32