PE anti-mouse CD365 (Tim-1) Antibody

Pricing & Availability
Clone
RMT1-4 (See other available formats)
Other Names
T cell immunoglobulin and mucin domain containing protein-1, T cell and airway phenotype regulator (Tapr), hepatitic virus cellular receptor 1, CD365
Isotype
Rat IgG2b, κ
Ave. Rating
3 reviews
Product Citations
publications
RMT1-4_PE_051105
Mouse Tim-1 transfected cells stained with anti-mouse CD365 (Tim-1, clone RMT1-4) PE.
  • RMT1-4_PE_051105
    Mouse Tim-1 transfected cells stained with anti-mouse CD365 (Tim-1, clone RMT1-4) PE.
See PE spectral data
Cat # Size Price Quantity Avail. Save
119505 50 µg 88€
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119506 200 µg 268€
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Description

CD365 (Tim-1) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-1 and hepatitis virus cellular receptor 1. It is developmentally expressed at high levels in the blastocyst. Tim-1 is expressed on activated CD4+ lymphocytes especially on Th2 cells and has been implicated to play a critical role in the development of atopic disease and other Th2-biased immune responses. Tim-1 is hepatitis A virus receptor in humans. Tim-4 is the endogenous ligand of Tim-1. The interaction of Tim-1 and Tim-4 is involved in costimulation of T cell proliferation.  Tim-1 is an endogenous ligand for LMIR5/CD300b.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance in each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
  1. Meyers JH, et al. 2005. Nat. Immunol. 6:455.
  2. Nakae S, et al. 2007. Blood doi:10.1182/blood-2006-11-058800.
  3. Yamanishi Y, et. al. 2010. J. Exp. Med. 207:1501. (FC)
  4. Namavari A, et al. 2012. Invest Ophthalmol Vis Sci. 53:4575. PubMed.
Product Citations
  1. Nagahara K, et al. 2008. J Immunol. 181:7660. PubMed
Publication Library
RRID
AB_2248167 (BioLegend Cat. No. 119505)
AB_2232887 (BioLegend Cat. No. 119506)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 33 kD.
Distribution

Developmentally regulated; highly expressed in embryonic development in blastocysts. Expressed on activated CD4+ lymphocytes, especially Th2 cell lines.

Function
Binds hepatitis virus, may play a critical role in immune responses and the development of atopic diseases (in particular airway hyperreactivity).
Ligand/Receptor
Binds hepatitis A virus in humans, Tim-4, LMIR5/CD300b
Cell Type
T cells, Th2
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. McIntire JJ, et al. 2001. Nature Immunol. 2:1109.
2. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
3. Wills-Karp M, et al. 2001. Nat. Rev. Immunol. 1:69.
4. Meyers JH, et al. 2005. Nat. Immunol. 6(5):455.

Gene ID
171283 View all products for this Gene ID
UniProt
View information about CD365 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01/14/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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