- 8B2 (See other available formats)
- Other Names
- IKAROS family zinc finger 3 (Ikzf3), zinc finger protein Aiolos, Zfpn1a3, Znfn1a3
- Mouse IgG
- Ave. Rating
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- Product Citations
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Aiolos, an Ikaros family member, is also known as Izkf3. It has a molecular weight of 58 kD and shows 70% homology to the Ikaros isoform, Ik-1, particularly in the DNA binding and C-terminal domains. Aiolos can form a heteromer with Ikaros or exist as a homodimer. The homomeric complex is a potent transcriptional activator. Unlike Ikaros, Aiolos is expressed in late fetal thymus and adult lymphoid organs. Among the adult hematopoietic progenitors, Aiolos is expressed at low levels in mulitpotent but not in pluripotent stem cells. When these multipotent progenitors become more restricted to T and B lymphoid pathways there is an upregulation in Aiolos expression. The highest expression of Aiolos is detected in the spleen (especially in B-cells) followed by thymus and bone marrow, and cannot be detected in the non-lymphoid organs. Aiolos plays an important role in lymphocyte proliferation and differentiation.Product Details
- Antibody Type
- Host Species
- N-terminal half of mouse Aiolos fused to GST
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, immunoprecipitation1, and Western blotting2.
Does not cross-react with human.
View more applications data for a different format of this clone in our Scientific Poster Library.
This product is for research use only, cannot be used on or in humans, and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact the Regents of the University of California.
- Application References
- Laszlo G, et al. 1993. J. Immunol. 150:5252. (ICFC, IP)
- Thompson EC, et al. 2007. Immunity 26:335. (WB)
- Product Citations
AB_2561700 (BioLegend Cat. No. 653203)
AB_2561701 (BioLegend Cat. No. 653204)
- 509 amino acids with predicted MW of 58 kD
Restricted to lymphoid lineage, expressed in B, NK and T cells
- Transcription factor that plays an important role in the regulation of lymphocyte differentiation; plays an essential role in regulation of B-cell differentiation, proliferation, and maturation to an effector state
- Homodimer and heterodimer with other IKAROS family members; interacts with IKZF4, IKZF5, IKZF1, HRAS, FOXP3, and BCL21L isoform Bcl-X(L); associates with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A
- Cell Type
- B cells, NK cells, T cells
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Innate Immunity, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Morgan B, et al. 1997. EMBO J. 16:2004.
2. Wang JH, et al. 1998. Immunity 9:543.
3. Cariappa A, et al. 2001. Immunity 14:603.
- Gene ID
- 22780 View all products for this Gene ID
- View information about Aiolos on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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