PE anti-human TMEM8A Antibody

Pricing & Availability
Clone
SA065C3 (See other available formats)
Regulatory Status
RUO
Other Names
M83, TMEM6, TMEM8, Transmembrane protein 8A
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
SA065C3_PE_TMEM8A_Antibody_1_040616
Human peripheral blood lymphocytes were stained with CD3-APC and TMEM8A (clone SA065C3) PE (top) or mouse IgG2b, κ PE isotype control (bottom).
  • SA065C3_PE_TMEM8A_Antibody_1_040616
    Human peripheral blood lymphocytes were stained with CD3-APC and TMEM8A (clone SA065C3) PE (top) or mouse IgG2b, κ PE isotype control (bottom).
  • SA065C3_PE_TMEM8A_Antibody_2_040616
See PE spectral data
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370805 25 tests 111€
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Description

TMEM8A is an 84 kD, type I protein with five transmembrane-spanning regions. TMEM8A is expressed on the surface of resting T cells and is down-regulated after cell activation. In addition, there are reports of TMEM8A co-localizing with LAMP2 in the lysosome. While the exact function of TMEM8A is unknown, it has been proposed to be an adhesion molecule that may be involved in the maintenance of the resting T cell state.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TMEM8A transfected cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone SA065C3 is also known as 5C31. Additional reported applications (for the relevant formats) include: immunofluorescence1 and Western blotting1.

Application References
  1. Lee GH, et al. 2016. J Cell Biol. 10.1083/jcb.201605121. (IF, WB) PubMed
RRID
AB_2616685 (BioLegend Cat. No. 370805)

Antigen Details

Structure
84 kD type I protein with five transmembrane-spanning regions.
Distribution

Resting T cells, down-regulated after cell activation.

Cell Type
T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Antigen References

1. Motohashi T, et al. 2000. Biochem. Biophys. Res. Commun. 276:244.
2. Schröder B, et al. 2007. Traffic 8:1676.
3. Palmieri M, et al. 2011. Hum. Mol. Genet. 20:3852.

Gene ID
58986 View all products for this Gene ID
UniProt
View information about TMEM8A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 10/29/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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