DyLight™ 649 Streptavidin

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Other Names
SAv-DyLightËäó 649
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Cat # Size Price Quantity Avail. Save
405224 100 µg 80€
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Description

Streptavidin binds to biotin with high affinity. DyLight™ 649-conjugated Streptavidin is useful for detecting biotinylated antibodies. The excitation of DyLight™ 649 by 654 nm laser light induces a fluorescence emission maximum of 670 nm.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
Streptavidin is conjugated with DyLight™ 649 under optimal conditions.
Concentration
0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IF - Reported in the literature

Recommended Usage

This streptavidin product is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the DyLight™ 649 conjugation. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Streptavidin-DyLight™ 649 is useful as a second step reagent for indirect immunofluorescent staining when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-DyLight™ 649 is 60 kD and Streptavidin alone is 52 kD.

Application References
  1. Van VQ, et al. 2012. PLoS One. 7:e41972. PubMed
  2. Zenker S, et al. 2014. J. Immunol. 192:2830. PubMed
Product Citations
  1. Van V, et al. 2012. PLoS One. 7:e41972. PubMed
  2. Zenker S, et al. 2014. J Immunol. 192:2830. PubMed
  3. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  4. Matsumoto S, et al. 2015. J Biol Chem. 290: 20071-20085. PubMed
  5. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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