Alexa Fluor® 647 anti-human TCL1 Antibody

Pricing & Availability
Clone
1-21 (See other available formats)
Other Names
T-cell leukemia/lymphoma protein 1A, Protein p14 TCL1, Oncogene TCL1, TCL-1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1-21_A647_110308.jpg
Human peripheral blood lymphocytes stained with anti-CD19 (HIB19) PE then intracellularly stained with 1-21 Alexa Fluor® 647
  • 1-21_A647_110308.jpg
    Human peripheral blood lymphocytes stained with anti-CD19 (HIB19) PE then intracellularly stained with 1-21 Alexa Fluor® 647
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
330508 100 tests 236€
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Description

TCL1, also known as T-cell leukemia/lymphoma protein 1A, protein p14 TCL1 and oncogene TCL-1, is a 14kD beta-barrel protein that was originally identified on the basis of its association with T-cell prolymphocytic leukemia (T-PLL). Activated by the T-cell receptor enhancer through inv(14)(q11;q32.1) or t(14;14)(q11;q32.1), TCL1 is normally expressed in immature cortical thymocytes, activated peripheral T-cells,  pro-B cells, and naive mantle zone B cells of peripheral lymphoid tissues, but not in post-germinal center (GC) memory B cells and plasma cells. Akt and TCL1 interact via Akt’s pleckstrin homology domain to form hetero-oligomers at the leaflet of the inner plasma membrane stimulating the protein kinase C-mitogen activated protein kinase-extracellular signal-related kinase (PKC-MAPK-ERK) signal transduction pathway. TCL1 also inhibits activation-induced cell death in T-cells by blocking PKCθ. In addition to T-PLL dysregulation is implicated in such B-cell neoplasias as lymphoblastic lymphoma, chronic lymphocytic leukemia (CLL) and Burkitt lymphoma.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
outer α-loop region of TCL1 peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunoprecipitation, western blot, immunohistochemical staining of frozen or formalin-fixed, paraffin embedded tissue sections.

Application References
  1. Herling M, et al. 2008. Blood 111:328
  2. Herling M, et al. 2007. Am. J. Surg. Pathol. 31:1123
RRID
AB_2204405 (BioLegend Cat. No. 330508)

Antigen Details

Structure
Beta barrel protein, 14kD
Distribution
Cytoplasm
Function
Enhances Akt mediated cell survival, stabilizes mitochondrial membrane potential
Interaction
Akt1, Akt2, Akt3, caco2
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1. Narducci MG et al. 2000. Cancer Research 60:2095.
2. Teitell MA et al. 2005 Nat. Rev. Cancer 5:640
3. Laine J et al.2000. Molecular Cell 6:395
4. Virgilio L et al. 1994. Proc. Natl. Acad. Sci. 91:12530
5. Masayuk N et al. 2007 FASEB J. 21:2273
6. Despouy G et al. 2007 Blood 110:4406
7. Rhine S et al. 2006 Blood 108:1991

Gene ID
8115 View all products for this Gene ID
UniProt
View information about TCL1 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/27/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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