Alexa Fluor® 647 anti-human CD171 (L1CAM) Antibody

Pricing & Availability
L1-OV198.5 (See other available formats)
Regulatory Status
Other Names
CAML1, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, S10, SPG1, L1CAM, Neural cell adhesion molecule L1
Mouse IgG2a, κ
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Product Citations
Human M21 cell line was stained with CD171 (clone L1-OV198.5) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ isotype control (clone MOPC-173) Alexa Fluor® 647 (open histogram).
  • 1_L1-OV198-5_A647_CD171_Antibody_052319
    Human M21 cell line was stained with CD171 (clone L1-OV198.5) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ isotype control (clone MOPC-173) Alexa Fluor® 647 (open histogram).
  • 2_L1-OV198.5_A647_CD171_Antibody_051818
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10mM, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permeabilized with PBS with 0.5% Triton X-100 and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 594 anti-Vimentin (clone O91D3) antibody (green) and Alexa Fluor® 647 anti-CD171 (clone L1-OV198.5) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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371607 25 µg 128€
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371608 100 µg 324€
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CD171, also known as L1CAM or L1, is a transmembrane glycoprotein. It is a 200-220 kD neuronal cell adhesion molecule and a member of the L1 protein family belonging to the immunoglobulin superfamily.

This cell adhesion molecule plays an important role in nervous system development, including neuron-neuron adhesion, signal transduction, axon guidance, cell migration and differentiation. CD171 also has a strong implication in treatment-resistanct cancers. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
SKOV-3 ovarian carcinoma cells.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
AB_2734424 (BioLegend Cat. No. 371607)
AB_2734425 (BioLegend Cat. No. 371608)

Antigen Details

200-220 kD. Several immunoglobulin-like domains and fibronectin-like repeats (type III), linked via a single transmembrane sequence to a conserved cytoplasmic domain.

Tetanus-toxin positive neurons, endothelial cells, certain epithelial cells, reticular fibroblasts, colon and breast carcinomas, colon melanoma, tumor cells of neuronal and mesothelial origin.

Adhesion molecule. Homotypic and heterotypic cell-cell interaction in neuronal myelination, neurite outgrowth and regeneration. Axonal guidance, neuronal migration.
Homophilic interaction with itself and heterophilic binding to integrins, other cell adhesion molecules, extracellular matrix molecules.
Cell Type
Endothelial cells, Epithelial cells, Fibroblasts, Neurons
Biology Area
Cancer Biomarkers, Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Wolterink S, et al. 2010. Cancer Res. 70(6):2504.
2. Huszar M, et al. 2006. Human Pathology 37(8):1000.
3. Kaifi JT, et al. 2006. Anticancer Res. 26(2A):1167.
4. Kaifi JT, et al. 2006. Mod. Pathol. 3:399.
5. Meier F, et al. 2006. Int. J. Cancer 119(3)549.
6. Stoeck A, et al. 2006. Biochem. J. 393:609.
7. Gutwein P, et al. 2005. Clin. Cancer Res. 11(7)2492.

Gene ID
3897 View all products for this Gene ID
View information about CD171 on

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Go To Top Version: 2    Revision Date: 05/23/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD171 (L1CAM)

  • PE anti-human CD171 (L1CAM)

  • Alexa Fluor® 647 anti-human CD171 (L1CAM)

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  • TotalSeq™-B0430 anti-human CD171 (L1CAM)


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