BioLegend's LEGENDplex™ bead-based immunoassays quantify multiple soluble analytes simultaneously in biological samples using a flow cytometer. The assay utilizes the same basic principles of sandwich immunoassays, whereby a soluble analyte is captured between two antibodies.
LEGENDplex™ kits are provided with predefined panels, ranging from 3 to 13 specificities, or customers can mix and match any subset within each predefined panel using our Mix and Match system. Custom options are also available.
Each kit provides sufficient reagents for at least 100 tests. The assay can be performed in a 96-well filter plate or v-bottom plate, allowing you to run 40 samples plus 8 standard curve titrations in duplicate, or they can be performed in FACS tubes, where duplicate titrations may not be necessary.
Software is downloaded for free and each kit comes with a dongle key required for use.